|Table of Contents|

Screening for Al-tolerant Rhizobium and Its Effects on Soybean-Rhizobium Symbiosis System(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年01期
Page:
105-111
Research Field:
Publishing date:

Info

Title:
Screening for Al-tolerant Rhizobium and Its Effects on Soybean-Rhizobium Symbiosis System
Author(s):
LI Xiu-ping 1; 2;  CHEN Shu-zhen 2;  HUANG Cong-bin 1;  NIAN Hai 2;  LIANG Shi-hua 1;  CHENG Yan-bo 2;  MU Ying-hui 2
（1. Guangdong AIB Polytechnic College, Guangdong 510507, China； 2. College of Agriculture/Guangdong Branch of National Center for Soybean Improvement, South China Agriculture University, Guangzhou 510642, China）
Keywords:
Soybean;  Acid-aluminum;  Rhizobium;  Symbiosis;  Nitrogen fixation
PACS:
S565.1；S157.4
DOI:
10.11861/j.issn.1000-9841.2018.01.0105
Abstract:
In order to solve the soil acidification problem of soybean planting in south China，the superior aluminum-tolerant rhizobia strains were screened by inoculation test, and find the special suitable rhizobial strains or Legume-rhizobium symbiotic nitrogen (N2) fixation (SNF) for acid soil in south China. The nodules of 20 cultivation soybeans which grew in Zengcheng and Huizhou of Guangdong province were isolated by using technique of strains activated culture method. The tolerance of aluminum was detected by spectrophotometer. The growth characteristics of aluminum-tolerant rhizobium were analyzed by using the method of inoculation. The effects of acid on growth and nodulation of the cultivated-soybean were explored by inoculating with aluminum-tolerant strains. The growth of YX30 strain on pH6.0 group was faster than the pH4.5 group under the same Al concentration. Four soybean genotypes inoculated with rhizobium of YX30 were cultured with different Al concentration at pH6.0 and pH4.5, respectively.Results indicated that when the Al concentration reached 200mol?L -1, the resistant genotype could nodulate while the sensitive genotype failed. As the concentration of Al increased, the number of nodules decreased. A concentration of 100mol?L -1 Al in the medium led to the negative growth of the dry weight of the plant shoots at both pH4.5 and pH6.0. In the medium with lower Al concentration（25 and 50mol?L -1）, nodule numbers were more than their controls at both pH4.5 and 6.0, activity of the N fixation enzyme, dry weight and N content of the shoots were higher than their corresponding controls. Although Al is an inhibited factor in acid environment, low concentration of Al could enhance nodulation, and increase the dry weight and N content of the shoots.

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Last Update: 2018-03-13