|Table of Contents|

Expression Patterns of Soybean Transcription Factor GmbZIP60 in Response to Abiotic Stresses(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年01期
Page:
45-49
Research Field:
Publishing date:

Info

Title:
Expression Patterns of Soybean Transcription Factor GmbZIP60 in Response to Abiotic Stresses
Author(s):
XING Jin-cheng 1;  SUN Chen-xi 2;  HONG Li-zhou 1;  XU Ling 3;  XU Zhao-long 3;  ZHANG Da-yong 3
(1.Institute of Agricultural Sciences in Coastal Area of Jiangsu,Yancheng 224002,China; 2.Shandong Academy for Environmental Planning,Jinan 250101,China; 3.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210094, China)
Keywords:
Soybean;  bZIP transcription factor;  Abiotic stress
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2018.01.0045
Abstract:
bZIP genes play significant roles in the response to abiotic stresses. We have cloned a bZIP gene named as GmbZIP60 (Genebank,Accession, No:DQ787038) from soybean, and transformed it into Arabidopsis thaliana. Then on the base of these, this paper studied the GmbZIP60 gene expression profiling in Arabidopsis seedlings under the treatments of PEG, high-temperature（37℃）, low-temperature（4℃）, high-salt(PEG6000) and ABA(100 mg·L -1) by qRT-PCR and GUS histochemical staining. The fluorescence quantitative RT-PCR results showed that the expression of GmbZIP60 genes were down-regulated by PEG, high-temperature, low-temperature and salt treatments respectively. The results of GUS staining were agree with fluorescence quantitative RT-PCR. This indicated that GmbZIP60 might be involved in the responsing to abiotic stresses such as high salinity, drought, high temperature and low temperature. This study laid the foundation for revealing the molecular mechanism of soybean resistance and genetic engineering improvement of soybean resistance.

References:

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Last Update: 2018-03-13