The T-DNA Flanking Sequence Analysis and Specific Detection Method of Transgenic Soybean with Gene HAL1(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2018年01期

Page:

32-38

Research Field:

Publishing date:

Info

Title:

The T-DNA Flanking Sequence Analysis and Specific Detection Method of Transgenic Soybean with Gene HAL1

Author(s):

CAI Qin-an;  SHANG Li-xia;  JIANG Zhi-lei;  YU Zhi-jing;  MA Rui

(Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, Changchun 130033, China)

Keywords:

Soybean;  Transgenic plant;  Flanking sequence analysis

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2018.01.0032

Abstract:

For the convenience of research and testing of HAL1 trasformed soybeans, thermal asymmetric interlaced PCR(TAIL-PCR) method were used to separate the T-DNA flanking sequence, with the T3 genomic DNA of HAL1 transgentic soybean as the template, by comparing the soybean genomic data, the T-DNA was inserted in soybean genome chromosome 1 non-coding region 49468395 loci as single copy. In order to ensure that the transgenic events do not affect the normal expression of the functional genes of the soybean genome. Under the threaten of 200 mmol?L ^-1 NaCl, the growth force of transgenic plant was significantly stronger than that of the control materials, protein quantitative test results showed protein in leaf reached to 0.03 mg?g ^-1, protein content in root, stem and flower was lower. Two pairs of specific detection primers were designed based on the two flanking sequences and the insertion of the transgenic plant, and two DNA fragments of 926 and 816 bp were acquired by PCR. In this study, the established T-DNA flanking sequences analysis will provide effective method in transgenic soybean detection and management.

References:

［1］ Clive J. 2015年全球生物技术/转基因作物商业化发展态势［J］. 中国生物工程杂志, 2016, 36(4): 1-11. (James C. Global biotechnology/GM crops commercialization development trend in 2015［J］. China Biotechnology,2016, 36(4): 1-11.)

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