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Study on Selection of SMV Resistance Genes in Soybean Germplasms and Segregation Populations using Molecular Marker(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2018年01期
Page:
14-21
Research Field:
Publishing date:

Info

Title:
Study on Selection of SMV Resistance Genes in Soybean Germplasms and Segregation Populations using Molecular Marker
Author(s):
LIU Shi-chao; REN Rui; WANG Li-qun; WANG Tao; LUO Jin-yan; ZHENG Huan-fang; ZHI Hai-jian; LI Kai
（Soybean Research Institute of Nanjing Agricultural University/Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, China）
Keywords:
Molecular markers;  Soybean;  Soybean mosaic virus;  Resistant genes;  Coincidence rates of selection
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2018.01.0014
Abstract:
Soybean mosaic virus (SMV) is one of the most important virus diseases in soybean production in China. In this study, 50 soybean germplasms from domestic and abroad along with the F2, F3 and F4 populations from the cross of “Kefeng 1 × RN-9” were evaluated and selected for the resistance to SMV strains SC15 and SC18 using the five molecular markers (Satt246, Satt286 and Satt634, BARCSOYSSR_02_0667 and Satt266) tightly linking to Rsc15 and Rsc18, which are soybean resistance genes to SC15 and SC18.The results in this study through phenotype confirmation revealed that the coincidental rates of selection of the molecular markers for Sat_246 and Satt286 to the resistance of SC15 were 63.89% and 66.67%, and that of Satt634, BARCSOYSSR_02_0667 and Satt266 to Rsc15 were 67.92%, 68.09% and 63.10%, respectively; 34 soybean germplasms with resistance to SC15, 27 to SC18 and 25 soybean germplasms with resistance to both SC15 and SC18 were obtained, respectively. The resistant gene of Rsc15 was selected in the F2, F3 and F4 populations using the molecular markers. The results showed that the coincidence rates of molecular markers for Sat_246 and Satt286 to Rsc15 in F2, F3 and F4 populations were 72.13%, 68.83% and 84.52%, respectively. The selection of resistance genes was performed in the F2, F3 and F4 populations using the 5 molecular markers linking to Rsc15 and Rsc18, and 18 homozygous lines with resistance to both SC15 and SC18 were obtained in F4. These results indicate that these molecular markers can be used effectively for the selection of resistance to SMV germplasm resources and in segregating populations.

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Last Update: 2018-03-13