|Table of Contents|

The Chemotaxis and Nodulation Effect of Soybean Rhizobium on Exogenous Flavonoids(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年06期
Page:
900-904
Research Field:
Publishing date:

Info

Title:
The Chemotaxis and Nodulation Effect of Soybean Rhizobium on Exogenous Flavonoids
Author(s):
AN Qi1; 2 ;  YIN Bo1; 2;  ZHANG Jie-chi1; 2;  YUAN Ming3;  WANG Lian-xia3;  YUAN Tao1; 2;  CAO Ya-bin1; 2;  NIU Yan-bo1; 2
（1.Institute of Microbiology，Heilongjiang Academy of Sciences，Harbin 150010，China； 2.Institute of Advanced Technology，Heilongjiang Academy of Sciences，Harbin 150020，China； 3.Qiqihar Branch Academy，Heilongjiang Academy of Agricultural Sciences，Qiqihar 161006，China）
Keywords:
Exogenous flavonoids; Rhizobium; Chemotaxis; Nodulation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.06.0900
Abstract:
In the study, Bradyrhizobium japonicum 2-39 was used as test strain Exogenous flavonoids such as daidzein, 4-methylumbelliferone, genistein and formononetin with different concentration (5, 10, 15, 20, 25 μg·mL-1) were selected as the inducer, respectively Aimed to study the chemotaxis effect of Bradyrhizobium japonicum 2-39 to exogenous flavonoids of different kinds and concentrations, capillary culture method was utilized to quantify the number of rhizobia colonies. The exogenous flavonoids which have significant effect on chemotaxis of Bradyrhizobium japonicum 2-39 and soybean genotype NF-16 were selected in the pot experiment to further explore the nodulation effect of soybean. The results indicated that daidzein and genistein exert more significant effect on Bradyrhizobium japonicum 2-39 compared with other group. The optimum concentration of daidzein and genistein was 15 μg·mL-1.?The results of pot experiment showed that simultaneous addition of daidzein and genistein could effectively stimulate soybean nodulation.

References:

［1］张武，杨琳，王紫娟. 生物固氮的研究进展及发展趋势［J］.云南农业大学学报，2015，30 （5）：810-821.（Zhang W，Yang L，Wang Z J. Advance and development trend of biological nitrogen fixation research ［J］. Journal of Yunnan Agricultural University，2015，30（5）：810-821.）

［2］关大伟，李力，岳现录，等. 我国大豆的生物固氮潜力研究［J］. 植物营养与肥料学报，2014，20（6）：1497-1504.（Guan D W， Li L，Yue X L， et al. Study on potential of biological nitrogen fixation of soybean in China ［J］. Journal of Plant Nutrition and Fertilizer，2014，20（6）： 1497-1504.）
［3］孟庆英，韩旭东，张春峰，等. 根瘤菌与微肥对大豆生理及产量的影响［J］. 黑龙江农业科学，2017，13（2）：51-54.（Meng Q Y，Han X D，Zhang C，et al. Effect of rhizobia and trace fertilizer on physiological and yield of soybean ［J］. Heilongjiang Agricultural Sciences，2017, 13（2）：51-54.）
［4］李欣欣，许锐能，廖红. 大豆共生固氮在农业减肥增效中的贡献及应用潜力［J］. 大豆科学，2016，35（4）：532-534.（Li X X，Xu R N，Liao H. Contributions of symbiotic nitrogen fixation in soybean to reducing fertilization while increasing efficiency in agriculture ［J］. Soybean Science，2016，35（4）：532-534.）
［5］Guan D W，Li L，Yue X L，et al. Study on potential of biological nitrogen fixation of soybean in China ［J］. Journal of Plant Nutrition and Fertilizer，2014，20（6）：1497-1504.
［6］Cooper J E. Multiple responses of rhizobia to flavonoids during legume root infection ［J］. Advances in Botanical Research，2004，41：59-62.
［7］Miao S J，Liu X B. Molecular physiology of nodulation and nitrogen fixation in soybean ［J］. Soybean Science，2010，29（2）：319-324.
［8］Chou M X，Wei X Y. Review of research advancements on the molecular basis and regulation of symbiotic nodulation of legumes ［J］. Chinese Journal of Plant Ecology，2010，34（7）：876-888.
［9］Cooper J E. Early interactions between legumes and rhizobia: Disclosing complexity in a molecular dialogue ［J］. Journal of Applied Microbiology，2007，103（5）：1-5.
［10］Redmond J W，Batley M. Flavones induce expression of nodulation genes in rhizobium ［J］. Nature，1986，4（23）：115-121.
［11］Geurts R，Federova E，Bisseling T．Nod factor signaling genes and their function in the early stages of rhizobium infection ［J］. Current Opinion in Plant Biology，2005，8（4）：346-352.
［12］Hartwing P，Maxwell C A，Joseph C M. Chrysoeriol and luteolin released from alfalfa seeds induce nod genes in rhizobium meliloti ［J］. Plant Physiology，1990，92 （1）：116-122.
［13］Irma V，Ton V B. The vetch (Vicia) and rhizobium leguminosarum bv-viciae symbiosis：A system to study the activity of rhizobium nod factors ［J］. Plant Molecular Biology，1994，21（15）：203-205.
［14］陈能场，黄维南. 黄酮类物质在豆科植物结瘤固氮中的作用［J］. 植物生理学通讯，1994，30（5）：368-370.（Chen N C，Huang W N. Role of flavonoide in nodulation and nitrogen fixation of legume plants ［J］. Plant Physiology Communications，1994，30（5）：368-370.）
［15］Firmin J L，Wilson K E，Rossen L，et al. Flavonoid activation of nodulation genes in rhizobium reversed by other compounds present in plants ［J］. Nature，1986，324（6）：90-92.
［16］Luo L，Yao S，Becker A，et al. Two new sinorhizobium meliloti Lys R.type transcriptional regulators required for nodulation ［J］. Journal of Bacteriology，2005，187（13）：4562-4572．
［17］欧阳平，张高勇，康保安. 类黄酮提取的基本原理、影响因素和传统方法 ［J］. 中国食品添加剂，2003，5（1）：54-57.（Ou Y P，Zhang G Y，Kang B A. The fundamental，affecting factors and traditional methods of the extraction of flavonoids ［J］. China Food Additives，2003，5（1）：54-57.）
［18］Han L，Wang X，Shu Q，et al. Identification and study on allelopathy of soybean root exudates ［J］. Soybean Science，2000，19（2）：119-125.

Memo

Memo:
-
Last Update: 2018-01-27