|Table of Contents|

Identification of IFS Transgenic Soybean and Selection of Plant with High -Isoflavones(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年06期
Page:
857-861
Research Field:
Publishing date:

Info

Title:
Identification of IFS Transgenic Soybean and Selection of Plant with High -Isoflavones
Author(s):
QI Yan-ping;  LIU Ya-jing;  XU Le-yi;  WANG Ling-yan;  PIAO Zhe-zhu;  CUI Guang-he;  YAN Fan; WANG Qing-yu
(College of Plant Science, Jilin University, Changchun 130062, China)
Keywords:
Soybean;  IFS;  Isoflavone content;  Three wavelength method
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.06.0857
Abstract:
As a healthy active substances, soybean isoflavones have obvious effects in the prevention of cancer, osteoporosis, cardiovascular disease and women′s climacteric syndrome IFS is one of the key enzymes in the pathway of isoflavone biosynthesis and it had a positive effect on the content of isoflavones in soybean seeds, but we still haven′t get stable and efficient transforms. Therefore, it is very important to improve the content of soybean isoflavones by studying isoflavone synthase pathway. In this study, after propagating IFS transgenic plants to T2generation, physiological indexes in transgenic plants had no obviously changes by agronomic traits analyzing.After identifying IFS transgenic soybeans by RT-PCR analysis, there were 60 IFS transgenic soybeans in 125 plants, accounting for 48%.This result indicated that IFS was stablily inherited in offspring.We used the modified three wavelength method to measure the isoflavone content and results showed that the average isoflavone content was 1.2 mg·g-1in IFS transgenic plants. Among the 41 IFSpositive plants, isoflavone content of 15 individual plants were higher than that of the non-genetically modified plants, which accounted for 36.6%.The IFS had a certain effect on the improvement of isoflavone content and it is possible to select plants with high isoflavones in IFS transgenic soybeans.This study clarified the effect of IFS on the content of isoflavones in transgenic soybean and obtained transgenic soybean containing much soybean isoflavones and provided an excellent germplasm resource for soybean breeding at the meantime. In additionally, the improved three wavelength method was more accurate and faster.

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Last Update: 2018-01-24