|Table of Contents|

Cloning and Expression Analysis of A RING/U-box Protein of Glyma.13G115900 from Soybean under Abiotic Stress(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年06期
Page:
851-856
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of A RING/U-box Protein of Glyma.13G115900 from Soybean under Abiotic Stress
Author(s):
ZHANG Yan-zheng; CHEN Long; LI Yong-guang; LI Wen-bin
(Key Laboratory of Soybean Biology in Chinese Ministry of Education/Agricultural Ministry′s Northeast Areal Key Laboratory of Soybean Biology and Genetic Breeding, Northeast Agricultural University, Harbin 150030, China)
Keywords:
Soybean;  RING/U-box protein;  Glyma.13G115900;  Gene cloning;  Stress response
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.06.0851
Abstract:
Based on the analysis of the transcriptome data of soybean drought stress, it was found that the soybean Glyma.13G115900 gene encodes a RING/U-box protein, and its expression level is affected by drought stress.In this study,Glyma.13G115900 gene was cloned from soybean.Amino acid multiplex sequence alignment indicated that the encoded protein had the same highly conserved RING/U-box domain as the other species.The prokaryotic expression vector pET-29b-Glyma.13G115900 was constructed and found to express Glyma13G115900 protein in Escherichia coli.Fluorescence quantitative PCR analysis showed that the expression level of Glyma.13G115900 gene was significantly affected by PEG, NaCl and ABA, and was not induced by cold stress.After treatment of PEG and NaCl, the expression level of this gene showed a significant decrease compared with CK, and the change of PEG expression was more obvious than that of NaCl, compared with CK, the mRNA abundance of the gene increased firstly and then decreased after ABA induction, and peaked at 4 h, suggesting that the gene may be involved in the abiotic response relying on the ABA pathway. The above results lay the foundation for further study of the gene.

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Last Update: 2018-01-24