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Effect of A Kind of Biological Stimulant on Microbial Community in Rhizosphere Soil of Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年05期
Page:
751-760
Research Field:
Publishing date:

Info

Title:
Effect of A Kind of Biological Stimulant on Microbial Community in Rhizosphere Soil of Soybean
Author(s):
YUAN Xin; WANG Meng-liang; WANG Jun-hong
（Institute of Applied Chemistry，Shanxi University，Taiyuan 03006，China）
Keywords:
Biostimulant;  Soybean;  Soil rhizosphere microbial;  DNA extraction;  Pyrosequencing
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.05.0751
Abstract:
In this paper, field experiments were carried out to study the influence of biostimulant (Diphenylurea Sulfonic- Calcium), developed by Shanxi University to the rhizosphere soil microbial community. Test set up five experimental groups, group S1 to S5, and one blank control group CK, then spray Chinese fisher Rhizobium or different concentrations of biostimulant to soybean seeds.Collected soybean rhizosphere soil during the flowering period, and compared the modified SDS method, TSAI method, Sangon magnetic bead kit and Mobile kit method, and selected the best Mobile Kit Method for soil bacterial DNA extraction.Amplified the fragment of 16S V3 region specifically, amplification products used for subsequent high-throughput sequencing analysis.The result showed that compared with the CK group, the Shannon values of the 5 experimental groups decreased. Compared with CK, S2, S3, S4 and S5 were found that the relative content of Proteobacteria, Actinomyces and Bacteroides were increased in the main phylum, and the relative contents of the phylum and the hard wall were reduced. At the genus level,it was also found that there was an increasing trend of functional bacteria in soybean growth and nitrogen fixation.Biostimulins made the diversity of soybean rhizosphere soil microorganism decreased, and could promote the growth of rhizobia, increase the content of rhizobia in rhizosphere soil microorganisms, at the same time, it could promote the number of functional bacteria in rhizosphere soil microbes, not only promote the soybean nitrogen fixation, but also promote the growth of soybean.

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Last Update: 2017-11-01