|Table of Contents|

Functional Prediction and Expression Analysis of Proline Synthetase Related Gene Glyma01g24530(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年05期
Page:
705-709
Research Field:
Publishing date:

Info

Title:
Functional Prediction and Expression Analysis of Proline Synthetase Related Gene Glyma01g24530
Author(s):
CHEN Wei;  WANG Tao;  LI Dong-mei;  LI Yue;  LI Yong-guang;  LI Wen-bin
(Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Breeding(Genetics) of Chinese Agriculture Ministry, Soybean Research Institute, Northeast Agricultural University, Harbin 150030, China)
Keywords:
Soybean;  Proline;  Glyma06g-28890;  Plant stress resistance
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.05.0705
Abstract:
Free proline can accumulate to high levels under drought-stress in many plants, and it is widely acknowledged that the increase of proline content could promote the plant resistant to drought stress.The study in Arabidopsis thaliana found that △1pyrroline-5-carboxylate synthetase (P5CS) catalyzed the rate.limiting enzyme in the biosynthesis of proline and played an important role in the biosynthesis of proline.In this study, function and expression of P5CS1 homologous gene in soybean Glyma01g-24530 was analyzed through bioinformatics and real-time quantitative PCR.We found that Glyma01g-24530 had conservative N.acetylglutamate synthase, kinase-like domain and Aldehyde dehydrogenase domain structure Phylogenetic tree showed that Glyma01g-24530 had high similarity with P5CS1 family members in many plants.We also found that there were multiple cis-elements about abiotic stress responses, light responsiveness in its promoter sequence region through the statistics of promoter cis-elements. Expression pattern analysis showed Glyma01g-24530 could be induced by drought and salt, it could express in many tissues.

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Last Update: 2017-10-29