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Genetic Transformation of Soybean with Drought Tolerance Gene cspB from Bacillus subtilis(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年05期
Page:
699-704
Research Field:
Publishing date:

Info

Title:
Genetic Transformation of Soybean with Drought Tolerance Gene cspB from Bacillus subtilis
Author(s):
SUN Xue-hui1CAI Qin-an2SHANG Li-xia2MA Rui2 YANG Xiang-dong2 YU Zhi-jing2WEI Jian1
(1.Changchun Normal University, Changchun 130032, China; 2.Agro-Biotechnology Research Institute / Jilin Provincial Key Laboratory Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China)
Keywords:
Soybean Drought tolerance cspB- gene Genetic transformation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.05.0699
Abstract:
To improve the drought tolerance of soybean, a vector pCAMBIA3301-cspB with drought tolerance related modified gene -cspB, was constructed.The modified-cspB gene was introducted into soybean variety Bert by Agrobacterium-mediated transformation. In this experiment total-3 800-cotyledonary node explants were infected and 95 independent transgenic plants were obtained by PCR and Southern blot analysis combined with PPT selection.The transformation efficiency was 2.5%. Seven transgenic plants with drought tolerance were selected,which could be used for soybean drought tolerance breeding in further.

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Last Update: 2017-10-29