|Table of Contents|

Mapping Stable QTLs for Growth Period Procedure Based on Four-way Recombination Inbred Line Population(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年05期
Page:
692-698
Research Field:
Publishing date:

Info

Title:
Mapping Stable QTLs for Growth Period Procedure Based on Four-way Recombination Inbred Line Population
Author(s):
NING Hai-long1;  ZHUANG Xu1;  YANG Chang1;  FANG Yan-long1;  WANG Ping1; 2;  LI Wen-bin1;  XUE Hong1;  LI Wen-xia1
(1.Agronomy Colledge, Northeast Agricultural University/Key Laboratory of Soybean Biology, Ministry of Education/Key Laboratory of Soybean Biology and Breeding（Genetics）, Ministry of Agriculture, Harbin 150030, China; 2.Information Center of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China)
Keywords:
Soybean;  Growth process;  Four-way recombination inbred line population;  QTL
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.05.0692
Abstract:
In this study, fourway recombinant inbred lines were constructed with Kenfeng 14, Kenfeng 15, Kenfeng 19 and Heinong 48 as the parents.The aim was to provide a theoretical basis and technical support for the molecular-assisted breeding of soybean growth stages, and to explore the genetic mechanism of genes related to growth period.QTL mapping was carried out at different stages of growth period in 2013, 2014 and 2015 under the three environments of Keshan and Harbin by 275 SSR molecular markers. The results showed that the growth stages of soybean four-way recombinant inbred lines were susceptible to environmental conditions, and the growth traits of the four-way RIL population had significant genetic variation.The QTLs associated with each stage of the growth period were distributed on 18 linkage groups, respectively, A1, A2, B1, B2, C1, C2, D1a, D1b, D2, F, G, H, J, K, L, M, N and O linkage groups;17 QTLs associated with each stage of the growth period were repeatedly mapped, respectively, qST-B2-1, qST-C1-1, qST-C2-1, qST-C2-2, qST-D1b-1, qST-D1b-2, qST-G-1, qST-H-1, qST-K-1, qST-K-2, qST-L-1, qST-L-2, qST-M-1, qST-M-2, qST-M-3, qST-N-1 and qST-O-1. Among them, 10 QTLs loci were newly found, qST-C2-2, qST-D1b-1, qST-K-1, qST-M-2 and qST-N-1 were localized in three or more environment or stages of growth period.

References:

［1］宁海龙, 白雪莲, 李文滨, 等. 大豆四向重组自交系群体蛋白质含量与油分含量QTL定位［J］. 作物学报, 2016, 42(11): 1620-1628. (Ning H L, Bai X L, Li W B, et al. Mapping QTL protein and oil contents using population from four-way recombinant inbred lines for soybean (Glycine max L Merr)［J］.Acta Agronomica Sinica, 2016, 42(11): 1620-1628.)

［2］Lee S H, Bailey M A, Mian M A R, et al. Molecular markers associated with soybean plant height, lodging, and maturity across locations［J］. Crop Science, 1996, 36(3), 728-735.
［3］宁海龙, 李琦, 李文滨, 等.大豆四向重组自交系群体遗传图谱的构建［J］. 大豆科学, 2015, 34(5): 776-781. (Ning H L, Li Q, Li W B, et al. Construction of population genetic map of soybean four-way recombinant inbred lines［J］. Soybean Science, 2015, 34 (5): 776-781).
［4］Fehr W R, Caviness C E. Stages of soybean development Special report 80, cooperative extension service, agriculture and home economics experiment station［J］. Iowa: Iowa State University, 1977，80: 11.
［5］Palomeque L, Liu L J, Li W, et al. QTL in mega-environments: I. universal and specific seed yield QTL detected in a population derived from a cross of high-yielding adapted × high-yielding exotic soybean lines［J］. Theoretical & Applied Genetics, 2009, 119(3): 417-427.
［6］Oyoo M E, Githiri S M, Benitez E R, et al. QTL analysis of net-like cracking in soybean seed coats［J］. Wetlands Ecology & Management, 2010, 18(6): 651-663.
［7］Orf J H, Chase K, Jarvik T, et al. Genetics of soybean agronomic traits: I. comparison of three related recombinant inbred populations［J］. Crop Science, 1999, 39(6): 1642-1651.
［8］Funatsuki H, Kawaguchi K, Matsuba S, et al. Mapping of qtl associated with chilling tolerance during reproductive growth in soybean［J］. Theoretical and Applied Genetics, 2005, 111(5): 851-861.
［9］Githiri S M, Yang D, Khan N A, et al. QTL analysis of low temperature induced browning in soybean seed coats［J］. Journal of Heredity, 2007, 98(4): 360-366.
［10］Wang D, Graef G L, Procopiuk A M, et al. Identification of putative qtl that underlie yield in interspecific soybean backcross populations［J］. Theoretical & Applied Genetics, 2004, 108(3): 458-467.
［11］Zhang W W Y, Luo G, Zhang J, et al. QTL mapping of ten agronomic traits on the soybean (Glycine max L Merr) genetic map and their association with EST markers［J］. Theoretical & Applied Genetics, 2004, 108(6): 1131-1139.
［12］Yamanaka N, Ninomiya S, Hoshi M, et al. An informative linkage map of soybean reveals QTLs for flowering time, leaflet morphology and regions of segregation distortion［J］. DNA Research, 2001, 8(2): 61-72.
［13］Sun S, Kim M Y, Van K, et al.QTLs for resistance to phomopsis seed decay are associated with days to maturity in soybean (Glycine max)［J］. Theoretical & Applied Genetics, 2013, 126(8): 2029-2038.
［14］Yamanaka N, Nagamura Y, Tsubokura Y, et al. Quantitative trait locus analysis of flowering time in soybean using a RFLP linkage map［J］. Breeding Science, 2000, 50(2): 109-115.
［15］Mansur L M, Orf J H, Chase K, et al. Genetic mapping of agronomic traits using recombinant inbred lines of soybean［J］. Crop Science, 1996, 36(5): 1327-1336.
［16］Mansur L M, Orf J, Lark K G. Determining the linkage of quantitative trait loci to RFLP markers using extreme phenotypes of recombinant inbreds of soybean (Glycine max L Merr)［J］. Theoretical & Applied Genetics, 1993, 86(8): 914-918.
［17］Mansur L M, Lark K G, Kross H, et al. Interval mapping of quantitative trait loci for reproductive, morphological, and seed traits of soybean (Glycine max L)［J］. Theoretical & Applied Genetics, 1993, 86(8): 907-913.
［18］Pooprompan P, Wasee S, Toojinda T, et al. Molecular marker analysis of days to flowering in vegetable soybean ［Glycine max (L) Merrill］［J］. Kasetsart Journal-Natural Science, 2006, 40(3): 2487-2489.
［19］Rossi M E, Orf J H, Liu L J, et al. Genetic basis of soybean adaptation to north american vs asian mega-environments in two independent populations from canadian × chinese crosses［J］. Theoretical & Applied Genetics, 2013, 126(7): 1809-1823.
［20］Komatsu K, Okuda S, Takahashi M, et al. Quantitative trait loci mapping of pubescence density and flowering time of insect-resistant soybean (Glycine Max L Merr)［J］. Genetics & Molecular Biology, 2007, 30(3): 635-639.
［21］Reinprecht Y, Poysa V W, Yu K, et al. Seed and agronomic qtl in low linolenic acid, lipoxygenase-free soybean［Glycine max (L) Merrill］ germplasm［J］. Genome, 2006, 49(12): 1510-1527.
［22］Guzman P S, Diers B W, Neece D J, et al. QTL associated with yield in three backcross-derived populations of soybean［J］. Crop Science, 2007, 47(1): 111-122.
［23］Khan N A, Githiri S M, Benitez E R, et al.QTL analysis of climatologist in soybean［J］.Theoretical & Applied Genetics, 2008, 117(4): 479-487.
［24］Kim K S, Diers B W, Hyten D L, et al.Identification of positive yield QTL alleles from exotic soybean germplasm in two backcross populations［J］. Theoretical & Applied Genetics, 2012, 125(6): 1353-1369.
［25］Bachlava E, Dewey R E, Burton J W, et al. Mapping and comparison of quantitative trait loci for oleic acid seed content in two segregating soybean populations［J］. Crop Science, 2009, 49(2): 433-442.
［26］Li D, Pfeiffer T W, Cornelius P L. Soybean QTL for yield and yield components associated with glycine soja alleles［J］. Crop Science,2008, 48(2): 571-581.
［27］Cheng L, Wang Y, Zhang C, et al. Genetic analysis and qtl detection of reproductive period and post-flowering photoperiod responses in soybean［J］. Theoretical & Applied Genetics, 2011, 123(3): 421-429.
［28］Eskandari M, Cober E R, Rajcan I. Genetic control of soybean seed oil: II-QTL and genes that increase oil concentration without decreasing protein or with increased seed yield［J］. Theoretical & Applied Genetics, 2013, 126(6): 1677-1687.
［29］Komatsu K, Hwang T Y, Takahashi M, et al. Identification of QTL controlling post-flowering period in soybean［J］. Breeding Science, 2012, 61(5): 646-652.
［30］Lee S H, Bailey M A, Mian M A R, et al. Identification of quantitative trait loci for plant height, lodging, and maturity in a soybean population segregating for growth habit［J］. Theoretical & Applied Genetics, 1996, 92(5): 516-523.
［31］Kabelka E A, Diers B W, Fehr W R, et al. Putative alleles for increased yield from soybean plant introductions［J］. Crop Science, 2004, 44(3): 784-791.

Memo

Memo:
-
Last Update: 2017-10-29