|Table of Contents|

Expression of GmWRKY35, a Soybean WRKY Gene, in Transgenic Tobacco Confers Drought Stress Tolerances(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年05期
Page:
685-691
Research Field:
Publishing date:

Info

Title:
Expression of GmWRKY35, a Soybean WRKY Gene, in Transgenic Tobacco Confers Drought Stress Tolerances
Author(s):
LI Da-hong;  WANG Chun-hong;  LIU Xi-ping;  WU Hai-yan;  LI Hong-yan
（School of Biotechnology and Food Engineering, Huanghuai University, Zhumadian 463000，China）
Keywords:
Glycine max L;  Tobacco;  GmWRKY35;  Drought stress
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.05.0685
Abstract:
Soybean, like other crops, suffers from drought, salt, low-temperature and other abiotic stresses, seriously resulting in yield decline. It is an effective way for crop breeding to improve resistance via molecular biology techniques. Zinc finger protein, an important transcription factor commonly found in plant, can regulate the expression of multiple stress-inducible genes and enhance comprehensive resistances effectively. In this study, a RING-H2 zinc finger protein gene, designated as GmWRKY35, was isolated from Glycine max L by RT-PCR. Its cDNA was 1 500 bp and encoded a putative protein of 499 amino acids with a predicted molecular mass of 54.89 kD and an isoelectric point（PI）of 6.74. A typical RING-H2 finger domain was found at the C-terminal region of GmWRKY35 protein.Subcellular localization analysis showed that the GmWRKY35 was expressed in nuclear.Real-time PCR showed that the transcript of GmWRKY35 was strongly induced by drought, salinity and cold to some extent. The GmWRKY35 gene was transformed into tobacco cultivar Wisconsin 38 （W38） by Agrobacterium-mediated under the control of the CaMV 35S promoter. Under drought stresses, transgenic tobacco lines carrying GmWRKY35 gene developed the strong primary root, smaller yellow leaves, higher POD and SOD activity, less MDA content and the percentage of electrolyte leakage, and performed higher tolerance to these stresses than the wide-type tobaccos. The primary function verification showed that overexpression of GmWRKY35 gene in tobaccos enhanced their tolerance to drought stresses. This study shows that RING-H2 zinc finger proteins of soybean play an important role in plant stress resistance and provide an excellent candidate for resistance breeding in soybean.

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Last Update: 2017-10-29