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Identification of Drought-tolerance of Soybean Germplasms from Yangtze and Huaihe River Valleys at Seedling Stage(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年05期
Page:
669-678
Research Field:
Publishing date:

Info

Title:
Identification of Drought-tolerance of Soybean Germplasms from Yangtze and Huaihe River Valleys at Seedling Stage
Author(s):
WANG Ying-dang;  XU Meng-ge;  ZHANG Ya-juan;  WENG Ye-yang;  LI Xiao-yong;  KONG Jie-jie;  ZHAO Tuan-jie;  HE Xiao-hong
(Soybean Research Institute/National Center for Soybean Improvement/Key Laboratory of Biology and Genetic Improvement/Key Laboratory of Biology and Genetic Improvement, Ministry of Agriculture/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China)
Keywords:
Soybean;  Seedling stage;  Drought tolerance;  Comprehensive evaluation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.05.0669
Abstract:
Abstract:The Yangtze and Huaihe River Valleys (YHRV) is an important area for soybean production in China. However, seasonal drought occurs frequently in this region, thus it is very necessary to identify and screen new drought-tolerant germplasms adapting to there. In the present study, 210 accessions of soybean germplasm, including 159 new breeding lines developed from YHRV and 51 parental lines, were planted in plastic pots under water-stressed and well-watered conditions in rainproof greenhouse in both 2015 and 2016.The drought tolerance coefficients of shoot dry weight (SDW), plant height (PH), tap root length (TRL), and root dry weight (RDW) at seedling stage were used as drought tolerant indices.Principle component analysis, the subordinate function value method and cluster analysis were jointly applied for comprehensive evaluation of drought tolerance for the tested genotypes.The results showed that the four traits were significantly decreased under the water-stressed condition compared with those under the well.watered condition.The averages for SDW, RDW, PH and TRL were reduced by 54%, 42%, 39% and 15%, respectively. The analysis of variance (ANOVA) showed that there was very significant differences between the two water treatments among the tested genotypes for all the traits, moreover, the first and the second order interactions of the three factors, i-e genotype, water treatment and year, for the PH and RDW were very significant.There was significant positive correlation relationship for drought-tolerant coefficients between SDW and PH, SDW and RDW, TRL and RDW, which reflected the inherent relations among the original drought tolerant indicators.The first three independent principal components extracted from principal component analysis accounted for 83.61% of the total variability and could adequately replace the original four indices which had overlapped information.To obtain the drought tolerance comprehensive evaluation D-value, the principal component scores for all the genotypes were then standardized through subordinate function and further average with corresponding eigenvalue as weight. All accessions were divided into five classes, i.e-high drought tolerance, drought tolerance, medium drought tolerance, drought susceptibility and high drought susceptibility, using-cluster analysis-based on D-value.Five-accessions (IA2077,YC4H/NN88.31//NN73.935,M8108,NN88.48/NN86.4and NN88.48/D76.1609)were identified as high drought tolerance and 57 was identified as drought tolerance among the total samples-27 lines (32.53% out of 83 genotypes in this region) from Southern YHRV and 19 lines (25.00% out of 76 genotypes in this region) from Northern YHRV were identified as high drought tolerance or drought tolerance.The screened genotypes could be used for drought tolerance breeding in soybean.

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Last Update: 2017-10-29