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Genetic Diversity and Population Structure Analysis of Sichuan Territory Soybean (Glycine max) and Introduced Resources by SSR Markers(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年05期
Page:
657-668
Research Field:
Publishing date:

Info

Title:
Genetic Diversity and Population Structure Analysis of Sichuan Territory Soybean (Glycine max) and Introduced Resources by SSR Markers
Author(s):
ZHONG Wen-juan;  YUAN Can;  ZHOU Yong-hang;  GONG Yi-yun;  JI Pei-cheng;  YANG Ze-hu;  MU Fang-sheng
(Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610300, China)
Keywords:
Glycine max;  Glycine soja;  SSR marker;  Genetic diversity;  Population structure
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.05.0657
Abstract:
In total, 53 SSR locus, covered the entire soybean genome with 2 to 5 SSR locus were chosen on each chromosome, were used to conduct the genomic scanning of a collection of 190 soybean germplasms.And then based on the markers experiment results to analyze genetic diversity, clustering, PCA and population structure.A total of 159 alleles were detected using 53 SSR markers while 3 alleles was detected on average per locus with the range 2.6, the average value of Nei’s gene diversity was 1. 474 4 with the range of 1.474 4±0.237 5, the polymorphism information content (PIC) was 0.305 with the range of 0.305 0±0.105 6, Shannon’s Information Index was 0.476 2 with the range of 0.476 2±0.124 9.These parameters indicated that the heterogeneous degree was not high and the genetic diversity was in the medium level in 190 soybean germplasms.The result of UPGMA cluster analysis indicated that the 190 soybean germplasms (Named P1) were divided into three major clusters.The 52 samples (Named P2) including 30 Sichuan soybean accessions, 14 abroad soybean resources and 8 wild soybean samples were divided into three broad clusters.Sichuan soybean accessions had a far genetic relationship with wild soybean and abroad soybean resources.The optimal K was 3 and 2 respectively in the population structure analysis of P1 and P2- P1 included 3 sub-populations: sub-population I contained 78 samples from different territory, sub-population II 59 germplasms had 7 wild soybean germplasms, sub-population III 53 samples only included 1 wild soybean zy05292- P2 had been divided into 2 sub-populations: sub-population I contained 24 Sichuan soybean accessions and 2 abroad resources, sub-population I had 26 germplasms including 8 wild soybean, 11 abroad resources and 7 Sichuan soybean accessions The selected 190 soybean germplasms were relatively highly diversified and variable in genetic analysis, revealing high genetic diversity. The result showed that the population structure of all resources was not strictly consistent with territory and original countries was credited to the wide genetic exchange in soybean. All the results indicated that we could make full use of abroad resources and upright wild soybean to innovate Sichuan soybean germplasm and to enrich Sichuan soybean genetic diversity.

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Last Update: 2017-10-29