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Hydrolysis Technology of Bean Dregs and the Hydrolysis Resistant Strains Screening(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年04期
Page:
620-625
Research Field:
Publishing date:

Info

Title:
Hydrolysis Technology of Bean Dregs and the Hydrolysis Resistant Strains Screening
Author(s):
SUN Chuan-bo;  ZHAO Qun;  ZHANG Chen;  HAN Bang-xing;  CHEN Cun-wu
（College of Biological and Pharmaceutical Engineering,West Anhui University, Liuan 237012, China）
Keywords:
Soybean residue;  Enzymolysis;  Cellulase;  Prandlase
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.04.0620
Abstract:
Abstract:Based on industrial production practice, this study discussed the efficient hydrolysis technology of bean dregs, breeding of yeast strains and high density cultivation process. Cellulase and prandlase hydrolyzed soybean residue were used in different technological conditions, the amount of sugar in the liquid was measured with glucose as the standard.The optimum technological conditions of hydrolyzed soybean residue were obtained as follows: 55℃, solid to liquid ratio 1∶24, hydrolysis firstly with pullulanase, adjusted to pH5.0, hydrolysis followed with cellulase, adjusted to pH5.5, hydrolysis 3 h with pullulanase and 1 h with cellulase, the time ratio was 3∶1, enzyme amount was 3%, enzyme ratio 1∶1, pullulanase∶cellulase=3∶1. This condition was used to hydrolyze soybean residue, the content of the material liquid contained the highest content of 160.7 mg·kg-1. Material liquid amount of 35% were used to culture resistant strain, the content of C yeast showed high resistance, contented yeast of 18 g·L-1

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Last Update: 2017-08-14