|Table of Contents|

Detection of Soybean Seed-borne Pathogens in Northeast China Using Loop-mediated Isothermal Amplification Assays(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年04期
Page:
592-597
Research Field:
Publishing date:

Info

Title:
Detection of Soybean Seed-borne Pathogens in Northeast China Using Loop-mediated Isothermal Amplification Assays
Author(s):
YUAN Yong-tian;  YE Wen-wu;  ZENG Dan-dan;  WANG Xiao-li;  WANG Yuan-chao;  ZHENG Xiao-bo
（Plant Protection College of Nanjing Agricultural University, Nanjing 210095, China）
Keywords:
Soybean disease;  Seed-borne pathogens;  LAMP(loop-mediated isothermal amplification);  Rapid detection of pathogen
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.04.0592
Abstract:
In order to know the situation of soybean seedborne pathogens in Northeast China, the seeds of 52 major soybean cultivars（lines） collected from this area were analyzed, using loop-mediated isothermal amplification(LAMP) assays for specific detection of 16 known soybean pathogen species, including .Colletotrichum gloeosporioide, C.truncatum, Calonectria ilicicola, Fusarium asiaticum, F.culmorum, F.equiseti, F. graminearum, F. oxysporum, F. proliferatum, F. solani, F. sambucinum, F. verticilliodes, Macrophomina phaseolina, Phomapsis longicolla, Phytophthora sojae, and Rhizoctonia solani. The result showed that there were 38 soybean cultivars(lines) carrying a total of 8 species of seed-borne pathogens, and the most frequent species was Phomapsis longicolla, followed by Rhizoctonia solani, Fusarium equiseti, F. asiaticum, F.graminearum, F. proliferatum, F. oxysporum and Colletotrichum truncatum.The number of detected species in each cultivar of seeds were between 1 and 4.In summary, a simple, rapid, and sensitive method for detecting soybean seed-borne pathogens was developed and successfully used to reveal the situation of soybean seed-borne pathogens in Northeast China.

References:

［1］刘志勇.大豆种子主要寄藏真菌及其分布［D］. 哈尔滨: 东北农业大学, 2012. (Liu Z Y. The main genraand distribution of seed-borne［D］. Harbin: Northeast Agricultural University, 2012.)

［2］马淑梅, 丁俊杰, 顾鑫, 等. 黑龙江省大豆主要病害发生危害调査［J］. 黑龙江农业科学, 2005(6): 48-52.(Ma S M, Ding J J, Gu X, et al. Survey on the occurrence and damage in main disease of soybean in Heilongjiang province［J］. Heilongjiang Agrcultrual Sciences, 2005(6): 48-52.)
［3］陆辰晨. 基于环介导等温扩增技术快速诊断大豆根部主要病害的研究［D］. 南京: 南京农业大学, 2015. (Lu C C. Rapid diagnosis of soybean root diseases based on Loop-mediated isothermal amplification(LAMP)［D］. Nanjing: Nanjing Agricultural University, 2015.)
［4］Souma J, Takeda N. Occurrence of soybean root necrosis caused by Calonectria ilicicola in Hokkaido［J］. 53rd Annual Report of the Society of Plant Protection of North Japan, 2002, 53:101-104.
［5］崔友林. 大豆茎枯病病原菌鉴定及3种检疫性大豆病原菌适生性分析［D］. 北京: 中国农业科学院, 2009. (Cui Y L. Identification of pathogen causing soybean stem blight and potential geographic distribution of three quarantine pathogens of soybean ［D］. Beijing: Chinese Academy of Agricultual Sciences, 2009.)
［6］魏思楠, 朱晓峰, 陈立杰, 等. 我国不同地区大豆主栽品种种子携带真菌研究［J］. 大豆科学，2014, 33(6): 890-895. (Wei S N, Zhu X F, Chen L J, et al. Seed-borne fungi detection in different soybean cultivars［J］. Soybean Science, 2014, 33(6): 890-895.)
［7］Ahmad I S, Reid J F, Paulsen M R, et al. Color classifier for symptomatic soybean seeds using image processing［J］. Plant Disease, 1999, 83(4): 320-327.
［8］Begum M M,Sariah M, Puteh A B, et al. Detection of seed-borne fungi and site of infection by Colletotrichum truncatum in naturally-infected soybean seeds［J］. International Journal of Agricultural Research, 2007, 2(9): 812-819.
［9］ShovanL R, Bhuiyan M K A, Sultana N, et al. Prevalence of fungi associated with soybean seeds and pathogenicity tests of the major seed-borne pathogens［J］. International Journal of Sustainable Crop Production, 2008, 3(4): 24-33.
［10］张仑, 殷幼平, 王中康. 环介导等温扩增技术的研究进展［J］. 贵州农业科学, 2009, 37: 21-25. (Zhang L, Ying Y P, Wang Z K. The advances and development of loop-mediated isothermal amplification method［J］. Guizhou Agricultural Sciences, 2009, 37: 21-25.)
［11］Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA［J］. Nucleic Acids Research, 2000, 28(12): E63-E63.
［12］Tian Q, Lu C C, Wang S S, et al. Rapid diagnosis of soybean anthracnose caused by Colletotrichum truncatum- using a loop-mediated isothermal amplification(LAMP) assay［J］. European Journal of Plant Pathology, 2017,148(4):785-793.
［13］曾丹丹, 张海峰, 田擎, 等. 基于环介导等温扩增技术检测黄淮地区大豆主栽品种种子携带的病原菌［J］. 南京农业大学学报, 2016, 39(6): 947-953. (Zeng D D, Zhang H F, Tian Q, et al.Detection of soybean seed-borne pathogens in Huang-huai area using LAMP assays［J］. Journal of Nanjing Agricultural University, 2016, 39(6): 947-953.)?
［14］李沐慧.东北地区大豆田间病害调查研究与鉴定［D］. 沈阳: 沈阳农业大学, 2016. (Li M H. Investigation and identification of soybean diseases in Northeast China［D］. Shenyang: Shenyang Agricultural University, 2016.)
［15］郑小波, 王帅帅, 陆辰晨, 等. 一种检测胶孢炭疽菌的环介导等温扩增引物组合物及其应用: 中国, 201510030095-6［P］. 2015-05-13. (Zheng X B, Wang S S, Lu C C, et al. A primer combination for detecting Colletotrichum gloeosporioide using loop-mediated isothermal amplification(LAMP) assays and its application: China, 201510030095-6［P］. 2015-05-136.)
［16］Xu M, Ye W W, Zeng D D, et al. Rapid diagnosis of wheat head blight caused by Fusarium asiaticum using a loopmediated isothermal amplification assay［J］. Australasian Plant Pathology, 2017,46(3):261-266.
［17］Zeng D D, Ye W W, Xu M, et al.Rapid diagnosis of soybean root rot caused by Fusarium culmorum using a loop-mediated isothermal amplification assay［J］. Journal of Phytopathology, 2017, 165(4): 249-256.
［18］Lu C C, Zhang H F, Wang Y C, et al. Rapid diagnosis of Fusarium root rot in soybean caused by Fusarium equiseti, or Fusarium graminearum, using loop-mediated isothermal amplification(LAMP) assays［J］. Australasian Plant Pathology, 2015, 44(4): 437-443.
［19］Lu C C, Dai T T, Zhang H F, et al. Development of a loopmediated isothermal amplification assay to detectFusarium oxysporum［J］. Journal of Phytopathology, 2015, 163(1): 63-66.［20］许苗, 叶文武, 王淑琛, 等. 快速检测马铃薯干腐病病原接骨木镰孢的环介导等温扩增技术［J］. 植物病理学报, 2017. DOI: 10.13926/j.cnki.apps.000038.(Xu M, Ye W W, Wang S C, et al. Rapid diagnose of potato dry rot caused by Fusarium sambucinum using a loop-mediated isothermal amplification assay［J］. Acta Phytopathologica Sinica, 2017. DOI: 10.13926/j.cnki.apps.000038.)［21］曾丹丹. 拟轮枝镰孢、黄色镰孢、雪松疫霉、栗黑水疫霉及大豆种传病原菌的LAMP检测［D］. 南京: 南京农业大学, 2017. (Zeng D D. LAMP detetion of Fusarium Verticillioids, F.Culorum, Phytophthora Lateralis, P. Cambivora and seedborne pathogens on soybean［D］. Nanjing: Nanjing Agricultural University, 2017.)［22］Lu C C, Song B, Zhang H F, et al. Rapid diagnosis of soybean seedling blight caused by Rhizoctonia solani and soybean charcoal rot caused by Macrophomina phaseolina using LAMP assays［J］. Phytopathology, 2015, 105(12): 1612［23］Dai T T, Shen H, Zheng X B. Establishment and evaluation of aTEF1α, based loopmediated isothermal amplification assay for detection of Phomopsis longicolla［J］. Australasian Plant Pathology, 2016, 45(3): 335-337.［24］Dai T T, Lu C C, Lu J, et al. Development of a loopmediated isothermal amplification assay for detection of Phytophthora sojae［J］. FEMS Microbiology Letters, 2012, 334(1): 27-34.

Memo

Memo:
-
Last Update: 2017-08-14