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Introduction of RNAi-PEPcGene into Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年04期
Page:
514-518
Research Field:
Publishing date:

Info

Title:
Introduction of RNAi-PEPcGene into Soybean
Author(s):
ZHANG Yuan-yu1;  DONG Ying-shan1;  YU Sheng-zhu2;  XING Guo-jie1;  LI Tong3;  ZHENG Hui-jing1;  ZHANG Ling1
(1.Jilin Academy of Agricultural Sciences, Jilin Provincial Key laboratory of Agricultural Biotechnology, Changchun 130033, China; 2. College of Resources and Environment, Northeast Agricultural University, Harbin 150030, China; 3.Life Science College of Northeast Normal University, Changchun 130024, China)
Keywords:
Soybean;  Transgenic;  pepc;  RNAi;  Cotyledonary node
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.04.0514
Abstract:
Abstract:Soybean is one of the most important oil seed crop planted as a major source of oil and protein for human. The cultivation of highoil soybean varieties has been one of the important issues for soybean breeding. In this study, we expressed the pepc-RNAi into the receptor varieties Shennong 9 by soybean cotyledonary nodes method.Though inhibiting the soybean endogenous GmPEPc gene expression to gain high oil of genetically modified soybean varieties.We co-cultivated about 407 explants, and got 35 transgenic plants, conversion rate 8.9%. Stock of glufosinate(300 mg·L-1) was painted on half the upper surface of T1?soybean leaves, and we had got 23 transgenic plants. PCR analysis showed that 12 of 14 transgenic plants were positive. Southern blot analysis of PCR-positive plants indicated the integration of the pepc-RNAi gene into the soybean genome.The results of T1?generation showed that the average oil content of transgenic soybean grain was 9.51% higher than that of the control, and the average protein content decreased by 5.44%. The results of these studies provide a basis for the selection of new varieties of high oil content, which provides the basis for the selection of new varieties of high oil.

References:

［1］Li R Z, Hatanaka T, Yu K, et al. Soybean oil biosynthesis: Role of diacylglycerol acyltransfersferases［J］. Functional & Integrative Genomics, 2013, 13(1): 99-113.

［2］Fire A, Xu S Q, Montgomery M K, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans［J］. Nature, 1998, 391: 806-811.
［3］李加瑞, 赵 伟, 李全梓, 等.Waxy基因的RNA沉默使转基因小麦种子中直链淀粉含量下降［J］. 遗传学报, 2005, 32(8): 846-854. (Li J R, Zhao W, Li Q Z, et al. RNA silencing of Waxy gene results in low levels of amylose in the seeds of transgenic wheat［J］. Acta Genetica Sinica, 2005, 32(8): 846-854.)?
［4］Pinto Y M, Kok R A, Baulcombe D C. Resistance to rice yellow mottle virus(RYMV) in cultivated African rice varieties containing RYMV transgenes［J］. Nature Biotechnology, 1999, 17: 702-707.?
［5］Feng Q, Qing Y, Wang C L, et al. Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice［J］. Euphytica, 2007, 158(2):35-45.?
［6］郭志鸿, 张金文, 王蒂, 等. 用RNA干扰技术创造高直链淀粉马铃薯材料［J］. 中国农业科学, 2008, 41(2): 494-501. (Guo Z H, Zhang J W, Wang D. Using RNAi technology to produce high-amylose potato plants［J］. Scientia Agricultura Sinica, 2008, 41(2): 494-501.)
［7］Sugimoto T, Tanaka K, Monma M, et al. Phosphoenolpyruvate carboxylase level in soybean seed highly correlates to its contents of protein and lipid［J］. Agricultural and Biological Chemistry, 1989, 53: 885-887.
［8］Sugimoto T, Kawasaki T, Kato T, et al. cDNA sequence and expression of a phosphoenolpyruvate carboxylase gene from soybean［J］. Plant Molecular Biology, 1992, 20: 743-747.
［9］陈锦清, 郎春秀, 胡张华, 等.反义PEP基因调空油菜籽粒蛋白质/油脂含量比率的研究［J］. 农业生物技术学报, 1999, 7(4): 316-320. (Chen J Q, Lang C X, Hu Z, et al. Antisense PEP gene regulates to ratio of protein and lipid content in brassica napus seeds［J］. Journal of Agricultural Biotechnology, 1999, 7(4): 316-320.)
［10］Olhoft P M, Somers D A. L.Cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells［J］. Plant Cell Reports, 2001, 20: 706-711.
［11］Olhoft P M, Flangel L E, Donovan C M, et al. Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method［J］. Planta, 2003, 216(5): 723-735.
［12］Sayaka H, Shinichiro O, Eri A, et al. The effects of spacer sequences on silencing efficiency of plant RNAi vectors［J］. The Plant Journal, 2001, 27(6): 581-590.
［13］吴关庭, 郎春秀, 胡张华, 等. 应用反义PEP基因表达技术提高稻米脂肪含量［J］. 植物生理与分子生物学学报, 2006, 32(3): 339-344. (Wu G T, Lang C X , Hu Z H, et al. Raising fat content in transgenic rice by anti-PEP gene transformation［J］. Journal of Plant Physiology and Molecular Biology, 2006, 32(3): 339-344.).
［14］赵桂兰, 陈锦清, 尹爱萍, 等. 获得转反义PEP基因超高油大豆新材料［J］. 分子植物育种, 2005, 3(6): 792-796. (Zhao G L, Chen J Q, Yi A P, et al. Transgenic soybean lines harbouring anti-PEP gene express superhigh oil content［J］. Molecular Plant Breeding, 2005, 3(6): 792-796.)

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Last Update: 2017-08-14