|Table of Contents|

Cloning and Transformation of the PAL2-3 Gene in Soybean (Glycine max L)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年04期
Page:
508-513
Research Field:
Publishing date:

Info

Title:
Cloning and Transformation of the PAL2-3 Gene in Soybean (Glycine max L)
Author(s):
LIANG Dong1;  XU Hong1;  QIN Hai-cheng1;  FAN Ge1;  WANG Xun2;  ZHANG Da-yong1
(1.Agronomy College of Northeast Agricultural University, Harbin 150030,China; 2.Biotechnology Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China）
Keywords:
Soybean;  PAL2-3 gene;  Recombinant vector;  Transgenic;  Soybean isoflavone
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.04.0508
Abstract:
Soybean isoflavones are a class of secondary metabolites synthesized in the phenylalanine metabolic pathway.Phenylalanine ammonia-lyase(PAL) is a key enzyme and rate-limiting enzyme in the phenylalanine metabolic pathway.In the preliminary study, we found that the relative expression of PAL gene and soybean isoflavone content had a significant synergistic trend, and it was found that PAL2-3(XM_003542493) was one of the major expression members with high relative expression in PAL gene family.In this study, we firstly cloned the PAL2-3 gene in soybean, then constructed the pCAMBIA3301-GmPAL2-3 plant overexpression vector and transformed itinto Agrobacterium tumefaciens EHA105.The transgenic plants were obtained by Agrobacterium tumefaciens-mediated transformation system, and the T1 transgenic plants were determined by PPT, exogenous marker gene Bar and quantitative PCR. The determination of isoflavones in soybean seeds was carried out on the-identified transgenic plants.The results showed that the expression level of PAL2-3 gene in T1?transgenic plants was 5.11-11.24 times of the control, and the highest of total isoflavones (2 587.63 μg·g-1) was 1.6 times of the control (1 616.90 μg·g-1).Therefore, overexpression ofPAL2-3 gene in soybean could increase the content of isoflavones in soybean seeds.

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Last Update: 2017-08-14