|Table of Contents|

Functional Prediction and Expression Analysis of GmABCG40(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年04期
Page:
502-507
Research Field:
Publishing date:

Info

Title:
Functional Prediction and Expression Analysis of GmABCG40
Author(s):
LI Yue1;  ZHANG Yu-hang1;  LI Dong-mei1;  WANG Tao1;  CHEN Wei1;  WANG Xun2;  LI Yong-guang1;  LI Wen-bin1
（Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Genetics Breeding of Chinese Agriculture Ministry/College of Agriculture of Northeast Agricultural University，Harbin 150030，China）
Keywords:
Soybean;  ABCG transporters;  Expression analysis;  Function;  Bioinformatics.
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.04.0502
Abstract:
GmABCG40 gene sequence was obtained by searching the homologous gene of AtABCG40 in the soybean genome database. Bioinformatics analysis of amino acid sequence encoded by GmABCG40 and promoter sequence showed that the full-length CDS sequence of GmABCG40 was 4 284 bp, encoding a 1 427 amino acids protein GmABCG40 was a hydrophobic protein, possessed multiple N-glycosylation sites,kinase phosphorylation sites, N-myristoylation sites, two ATP/AGP binding site motif A and one tachykinin family signal. Structure domains analysis indicated that GmABCG40 contained two nucleotide binding domains and two trans membrane domains, which constituted NBD1-TMD1-NBD2-TMD2 structure and belonging to the ABCG subfamily.Cis-elements associated with hormones, stresses, light responses, endosperm expression and transcription factors binding were existed in the predicted promoter region.Phylogenetic analysis results suggested that GmABCG40 had the highest genetic relationship with leguminous plants such as Phaseolus vulgaris, Vigna angularis, Cajanus cajan and Lotus japonicus.Results of tissue specific expression analysis revealed that expression level of GmABCG40 was lowest in leaves, however, highest in roots, it was speculated to participate in the ABA transport process in roots.

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Last Update: 2017-08-14