|Table of Contents|

Gene Mapping and Character Performance of A Yellow-green Leaf Mutant NJ9903-5 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年04期
Page:
494-501
Research Field:
Publishing date:

Info

Title:
Gene Mapping and Character Performance of A Yellow-green Leaf Mutant NJ9903-5 in Soybean
Author(s):
KONG Ke-ke;  XU Meng-ge;  WANG Ya-qi;  KONG Jie-jie; Al-Amin G M;  ZHAO Tuan-jie
(Soybean Research Institute， Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory for Biology and Genetic Improvement of Soybean (General), Ministry of Agriculture/National Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing 210095，China)
Keywords:
Soybean(Glycine max L);  Chlorophyll deficient mutant;  Morphological characteristics;  Genetic analysis;  Gene mapping
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.04.0494
Abstract:
Leaf color mutants are specific experimental materials in the study of chlorophyll synthesis, degradation and photosynthesis and can also be used as a marker trait in crop breeding.The results of the study on a spontaneous yellow-green seedling mutant NJ9903-5 showed that the young leaves from the opposite unifoliolate at VC growth stage to each trifoliolate ones exhibited yellow and gradually turn to green till mature. The number of chloroplast in yellow leaves decreased with less and loosely arranged stroma lamellae. Moreover, the contents of chlorophyll a, b and carotenoid significantly decreased. A large number of variations of seven agronomic and quality traits were found in two F2?populations from the crosses between NJ9903-5 and normal parents.The mutant F2?plants had significantly low values of plant height, number of nodes on main stem, pods and seeds number per plant in comparison with normal plants, while no significant differences were detected for 100-seed weight, protein and oil content.Genetic analysis revealed that the mutant trait was controlled by a recessive nuclear gene designated as ygl.The gene was mapped in a physical interval of 366 kb region between SSR markers BARCSOYSSR_02_1445 and BARCSOYSSR_02_1477 on chromosome 2.Among the 36 annotation genes in the region, Glyma.02G233700, a predicted chloroplast membrane transport protein, was identified.Sequencing analysis showed that a deletion of G nucleotide at the 38 base of exon 1 was detected in the NJ9903-5, and the frame shift mutation of Glyma.02G233700 can lead to a premature termination of the protein translation.

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Last Update: 2017-08-14