|Table of Contents|

The Characteristic of Bacterial Community Diversity in Soybean Field with Continuous Cropping Based on the High-Throughput Sequencing(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年03期
Page:
419-424
Research Field:
Publishing date:

Info

Title:
The Characteristic of Bacterial Community Diversity in Soybean Field with Continuous Cropping Based on the High-Throughput Sequencing
Author(s):
ZHU Lin1;  ZENG Chun-lin1;  LI Yu-qing1;  YU Bing-qian2;  GAO Feng1;  WEI Wei2;  XU Yan-li3
(1.School of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, China; 2. Jiangsu Provincial Key Laboratory of Modern Agricultural Equipment and Technology, Zhenjiang 212013, China; 3. Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150081, China)
Keywords:
Soybean;  Long-term continuous cropping;  Miseq;  Bacteria community;  Composition diversity
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.03.0419
Abstract:
Based on the Miseq platform of Illumina company, we explored the effect of short-term and long-term copping continuous with soybean on the composition diversity of the bacterial community.According to the sequencing of the V4 region of bacterial 16S rRNA gene, we obtained 180 980 and 221 424 efficient sequences, respectively, and confirmed them as 1 254 and 1 432 OTUs. The richness and diversity of bacterial community form the soybean field with long-term continuous cropping was higher than those in soybean field with short-term continuous cropping.At the phylum level, there were 8 bacterial phylum as the dominant group in both fields(the ratio to total above 1%), including Proteobacteria, Acidobacteria, Verrucomicrobia, Firmicutes, Bacteroidetes, Actinobacteria, Gemmatimonadetes and Nitrospira. All of which were 85.5% and 86.3% of the total community in short and long-term continuous cropping fields, respectively. At the genus level, the top 10 groups were the same bacterial genera between the both treatments, including Spartobacteria in Verrucomicrobia, Gp1, Gp4, Gp3 and Gp6 in Acidobacteria, Gemmatimonas in Gemmatimonadetes, Nitrospira in Nitrospira, Sphingomonas and Bradyrhizobium in Proteobacteria, and Bacillus in Firmicutes. All of these genera were 71.3% and 69.0% of the total community in each field, respectively.The results of this study indicated that the richness and diversity of bacterial community in soybean field was increased after long-term continuous cropping, although there was just a little change in bacterial community composition, especially those of dominant groups.It is of great significance to explain the mechanism of the formation of suppressive soil for soybean root disease by long-term continuous cropping.

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Last Update: 2017-06-28