|Table of Contents|

Symbiotic Compatibility Among Eight Elite Soybean Rhizobia Strains and Twenty-seven Soybean Cultivars from Different Planting Regions(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年03期
Page:
405-418
Research Field:
Publishing date:

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Title:
Symbiotic Compatibility Among Eight Elite Soybean Rhizobia Strains and Twenty-seven Soybean Cultivars from Different Planting Regions
Author(s):
WU Hui;  ZHONG Zhe-dong;  FAN Wei;  PENG Ya-qi;  DU Si;  CHEN Da-song;  WANG Xue-lu;  LI You-guo
(Huazhong Agricultural Univerity, College of Life Science and Techonology/State Key Laboratory of Agricultural Microbiology, Wuhan 430070, China)
Keywords:
Elite rhizobia strain;  Glycine max;  Soybean major cultivar;  Compatibility;  Symbiotic nitrogen fixing efficency
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.03.0405
Abstract:
Soybean is an important oil crop as well as a high-protein grain and forage crop, possessing symbiotic nitrogen fixation ability with rhizobia. Over the last decade, China′s new soybean varieties have been updated quickly, resulting in its poor matching with lag-behinded soybean rhizobia strains and therefore demonstrating null efficiency with inoculated rhizobia in agricultural field.To study on the compatibility and matching ability between rhizobia and soybean varieties, and screen the elite rhizobia strains with high nitrogen fixation efficiency and a broad-host range is of importance. It can provide elite strains and practical guides for rhizobia inoculant production and application in a specific region of China. This paper selected eight soybean rhizobia strains including six fast-growing soybean rhizobia previously isolated in our lab and two slow-growing soybean rhizobia .Bradyrhizobium japonicum, TA11 and USDA110, as controls, and twenty-seven soybean cultivars to carry out the matching experiments. The compatibilities between soybean rhizobia and soybean cultivars were characterized through pot sand nodulation experiments.Plant shoot biomass and height above ground, nodule number, fresh weight and nitrogenase activity were measured, and the data were analyzed using SPSS. It showed that nodule nitrogen fixing efficiency was significantly different among the tested rhizobia and soybean cultivars. Plant biomass was positively correlated with other indexes. Eight tested rhizobia were found to be compatible with twenty-four tested soybean cultivars in China. Plant biomass was significantly increased for inoculated plants relatived to that of the non-inoculated ones.In addition, the strains of HN01, GR3, HH29 and HH103 demonstrated high nitrogen fixation efficiency no less than that of USDA110. These strains are potential merit strains to be applied in Norheast China, Valley of the Yellow and Huaihe Rives, Valley of the Yangze River, and Southeast China.Among the twenty-seven soybean varieties/lines, Zhongdou 39, BD2 and Tianlong 1 showed higher biomass when inoculated with eight tested rhizobia. In addition,we have obtained an outstanding pair of with higher symbiont efficient Zhongdou 39-GR3, which will be applied in Valley of the Yangze River; the best compatibility of soybean-rhizobia in Norheast China, Valley of the Yellow and Huaihe Rives, Southeast China respectively were Liaodou 14-HN01, Xudou 14-HN01, BD2 HN01 as well. Our study elementary identified the compatibility between elite soybean rhizobia tested and major cultivar of different planting region in China.The results provide the rhizobia resources for inoculant production being supposed to match a region-specific soybean cultivar. Further investigations are need to evaluate and confirm the elite rhizobia strains that can compete with indigenous rhizobia strains and demonstrate practical higher nitrogen fixation efficency in the field soils.

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Last Update: 2017-06-28