|Table of Contents|

Transgenic Identification of Pod-shattering Gene GmAGL8 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年03期
Page:
360-364
Research Field:
Publishing date:

Info

Title:
Transgenic Identification of Pod-shattering Gene GmAGL8 in Soybean
Author(s):
QIN Yu-tao1;  WANG Rui2;  LYU Jin-dong1;  TIAN Yuan2;  HUANG Tian-long2;  XIE Hao1;  GUO Bei2; 3
(1.College of Plant Science and technology, Beijing University of Agriculture, Beijing 102206, China; 2.College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China; 3.Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture, P R, Beijing 102206, China)
Keywords:
Soybean;  Pod-shattering;  GmAGL8;  Transgene
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.03.0360
Abstract:
Pod shattering is a natural characteristic of soybean, which is one of the critical factors influencing production. In our previous study, we had transformed the gene GmAGL8(which putative related to soybean pod-shattering) into Glycine max and obtained the T1generation.In this study, the T 2 and T3 generation were multiplied by the T 1 generation carrying the GmAGL8-gene.The genetic stability and expression of transgenic plants were analyzed by using PCR and RT-qPCR methods. As a control group, wild-type Zhonghuang 10 was used to analyze and identify the traits of soybean pods.The results showed the positive rate of the GmAGL8 gene in T1, T2 and T 3 generation were 87.5%, 100% and 100% respectively.It demonstrated that the GmAGL8 gene can be inherited stably.The results of RT-qPCR analyzing showed the relative expression amount of GmAGL8-gene in transgenic plants was significantly higher than that in non-transgenic plants, and the amounts were various among the transgenic plants. The statistical results for pod-shattering among all plants indicated that the rates of pod-shattering were different among transgenic plants and non-transgenic plants, the average rate of pod shattering among transgenic plants of all generations was 9.09%, the rate of pod shattering among non-transgenic plants was 83.3%.In conclusion, GmAGL8 gene can be inherited stably and expressed normally among transgenic soybean.Furthermore, the phenotypic identification results proved the GmAGL8 gene is related to pod-shattering.

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Last Update: 2017-06-24