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Haplotype Analysis and Molecular Marker Development of Soybean Nematode Cyst Resistance Loci -rhg1 and Rhg4 in Soybean Germplasm(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年03期
Page:
345-350
Research Field:
Publishing date:

Info

Title:
Haplotype Analysis and Molecular Marker Development of Soybean Nematode Cyst Resistance Loci -rhg1 and Rhg4 in Soybean Germplasm
Author(s):
SHENG Bi-han;  LIU Bing;  CHEN Xiu-lan;  ZHU Zhi-jia;  TAN Yun-fu;  LI Wen-bin;  ZHAO Xue;  HAN Ying-peng
（Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybear Biology and Breeding (Genetics) of Chinese Agriculture Ministry, Northeast Agricultural University, Harbin 150030, China）
Keywords:
Soybean cyst nematode;  rhg1;  Rhg4;  SSR;  SNP
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.03.0345
Abstract:
Soybean cyst nematode (SCN), also named verticillium wilt, is a kind of soil spread parasitic disease.SCN disease is caused by soybean cyst nematode infection on soybean roots and seriously threats to the soybean production rhg1 and Rhg4 were the main loci for resistance to soybean cyst nematode (SCN). SSR and SNP markers tightly linked with rhg1 and Rhg4 loci were used to genotype fifteen soybean germplasm.The positive rates of resistance germplasm for Satt309 which was linked with rhg1 loci was 55.56% and that for Sat_162 which was linked with rhg1 loci was 66.67%.The sequencing result showed that the adenine/cytosine (A/C) mutation existed at 630 bp location of the key fragment at rhg1 loci. The disease-susceptible allele was base ‘A’ while the disease-resistant allele was ‘C’. The cytosine and guanine (C/A) mutation existed at 2 749 bp location of the key gene SHMT of Rhg4 loci. The disease-susceptible allele was base ‘C’ while the disease-resistant allele was ‘G’.The positive rates of resistance germplasm for SNP markers in rhg1 and Rhg4 loci were 77.78% and 100%, respectively. The genotyping method based on high resolution melting curve (HRM) for SNP markers of rhg1 and Rhg4 loci was established.The ideal genotyping results were obtained when the condition of scanning was starting at 65℃, holding at 60℃ and ending at 94℃.

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Last Update: 2017-06-24