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Salt Tolerance Analysis of GmGT2B Transgene Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年02期
Page:
213-219
Research Field:
Publishing date:

Info

Title:
Salt Tolerance Analysis of GmGT2B Transgene Soybean
Author(s):
LI Dongmei;  ZHOU Shuli;  LI Yongguang;  ZHANG Binbin;  LI Wenbin
（Key Laboratory of Soybean Biology in Chinese Ministry of Education/Agricultural Ministry’s Northeast Areal Key Laboratory of Soybean Biology and Genetic Breeding, Northeast Agricultural University, Harbin 150030, China）
Keywords:
GmGT2B;  Drought soybean;  SOD;  MDA;  Proline
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.02.0213
Abstract:
Using potted experimental method, we treated the GmGT2Btransgene soybean and the control with 200 mmol?L-1salt stress in seedling stage, and then measured their damage degree, superoxide dismutase(SOD) activity, malondialdehyde(MDA), proline, K+, Na+ content and the changes of gene expression. As a result, after treated with salt stress for 5 days, injury of leaves in transgenic soybean were lighter than the control under salt stress, and the SOD activity, proline content and malondialdehyde(MDA) levels were also significantly or extremely different; Na+content of soybeans increased as salt stress time increased, the N11, N24 of the Na+content reached twice of the control after 8 days under salt stress; GmGT2B gene expression of N11, N22 and N24 were increased and arrived the peak in the 8 day. Gene expression and variation tendency were consistented with physiological indicators, which were SOD activity and proline and K+, Na+content. The result indicated that GmGT2B gene was related to the responses of salt, the extent of injury of GmGT2B transgene soybean were lower than the control with N11 and N24 salt sress, GmGT2B gene and the transgene soybean both had a large breeding value.

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Last Update: 2017-05-14