|Table of Contents|

The Authentic Evaluation on Hybrids of Wild Soybean and Cultivated Soybean Based on SRAP Marker in Inner Mongolia(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年02期
Page:
193-199
Research Field:
Publishing date:

Info

Title:
The Authentic Evaluation on Hybrids of Wild Soybean and Cultivated Soybean Based on SRAP Marker in Inner Mongolia
Author(s):
LIU Xuejiao; WANG Mingjiu; SUO Rongzhen
（College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010018,China）
Keywords:
SRAP marker; Hybrids; Primer screening; Hybrids identification
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.02.0193
Abstract:
In this study，the hybrids of wild soybean and cultivated soybean were analyzed by SRAP marker to select out the true hybrids and build fingerprint15 primer combinations of SRAP with characteristics of rich polymorphism, clear bands and good repeatability were selected from 234 primer combinations at first, then primers were selected to bands specific for the male parent but absent from the female, 10 primers were selected and applied to identify the hybrids There were 48 progenies with those specific bands, which were therefore identified as true hybrids The 15 primer combinations were used in the PCR amplification，347 DNA bands were obtained, 265 of which bands were polymorphic, amounted to 76-37% of the total amplified bands The result showed that SRAP markers could be used as effective molecular markers for the authenticity identification of the progenies of soybean, the selected 15 primer combinations could be effectively applied to SRAP analusis of the germplasm resources of hybrids

References:

［1］姜国军,高利国,王玉芝野大豆引种栽培［J］.中国林业, 2011(23)：39（Jiang G J, Gao L G, Wang Y Z Wild soybean cultivation［J］. Chinese Forestry,2011(23):39.）

［2］燕雪飞中国野大豆遗传多样性及其分化研究［D］. 沈阳：沈阳农业大学，2014（Yan X F Genetic diversity and differentiation of the wild soybean (Glycine soja Siebet Zucc) in China ［D］. Shenyang: Shenyang Agricultural University,2014）
［3］范虎，赵团结，丁艳来，等中国野生大豆群体特征和地理分化的遗传分析［J］. 中国农业科学,2012,45(3):414-425（Fan H, Zhao T J, Ding Y L, et al Genetic analysis of the characteristics and geographic differentiation of chinese wild soybean population［J］. Scientia Agricultura Sinica,2012,45(3):414-425）
［4］黄仁术野大豆的资源价值及其栽培技术［J］. 资源开发与市场,2008,24(9):771-814．（Hang R S Resources value and cultivation techniques of Glycine soja［J］. Resource Development &Market, 2008,24(9):771-814．）
［5］刘传才，耿世刚，魏国印，等秦皇岛市野大豆保护措施研究［J］. 中国环境管理干部学院学报，2014,24(2):27-29 (Liu C C, Geng S G, Wei G Y, et al A study on protection measures of wild soybean (Glycine soja) in Qinghuangdao City［J］. Journal of EMCC，2014,24(2):27-29)
［6］冯光海野大豆在黄河三角洲的现状及保护措施［J］. 中国林业,2007(3B):39(Feng G H Present situation and protection of wild soybean in the Yellow River delta［J］. Chinese Forestry,2007(3B):39)
［7］王丹阳,吴铭对黑龙江省野大豆保护与利用的研究［J］林业勘查设计,2011(1):8586(Wang D Y, Wu Y Study on protection and utilization of wild soybean in Heilongjiang Province［J］. Forest Investigation Design,2011(1):85-86)
［8］姜海英,崔明元,周柏明,等浅谈北方春播大豆杂交育种 ［J］. 内蒙古农业科技, 2011（5）：124-125 (Jiang H Y, Cui M Y, Zhou B M, et al Introduction to the north spring sowing soybean cross breeding［J］. Inner Mongolia Agricultural Science And Technology, 2011（5）：124-125 )
［9］高淑芹,赵婧,蔡蕾, 等野生大豆种质资源及其创新利用［J］.农业与技术,2010,30(4):27-28 (Gao S Q, Zhao J, Cai L, et al Wild soybean germplasm innovation and utilization of resources［J］. Agriculture &Technology,2010,30(4):27-28)
［10］陈丽丽,王明玖,何丽君,等内蒙古野生大豆与栽培大豆杂交后代农艺性状比较及聚类分析［J］.中国油料作物学报，2013，35(2):142-147(Chen L L, Wang M J, He L J, et al Agronomic characteristics and cluster analysis of hybrids of Glycine soja Sieb．et Zucc．and Glycine．max(L．) Merr． in Inner Mongolia［J］. Chinese Journal of Oil Crop Sciences，2013，35(2):142-147)
［11］陈丽丽野大豆与栽培大豆杂交后代的鉴定评价研究［D］.赤峰：内蒙古农业大学，2013(Chen L LStudy on identification and evaluation of the lines from the cross of Glycine soja and Glycine max［D］. Chifeng: Inner Mongolia Agricultural University,2013)
［12］马晓萍,杨光宇,杨振宇,等野生大豆在大豆育种中的应用［J］. 作物研究,2009,23(1):11-12(Ma X P, Yang G Y, Yang Z Y, et al The application of wild soybean in soybean breeding［J］. Crop Research,2009,23(1):11-12)
［13］刘承源,王辉,邱文昌,等基于过氧化物同工酶分析月季种质资源的亲缘关系及杂种真实性［J］.广西植物, 2016,36(1):114-120(Liu C Y, Wang H, Qiu W C, et al Genetic relationships and hybrids reality in rose germplasm based on POD isozyme［J］. Guihaia, 2016,36(1):114-120)
［14］钟淮钦,李富生,杨清辉甘蔗杂种真实性鉴定研究综述［J］.热带农业科学, 2005,121(6):390-394(Zhong H Q, Li F S, Yang Q H A summary on sugarcane hybrid reliability identification［J］.Chinese Agricultural Science Bulletin, 2005,121(6):390-394)
［15］刘昔辉,方锋学,高轶静,等斑茅割手密杂种后代真实性鉴定及遗传分析［J］.作物学报,2012,38(5): 914-920 (Liu X H, Fang F X, Gao Y J, et al Identification and genetic analysis of hybrid from cross between Erianthus arundinacius (Retz) Jesws and Saccharum spontaneum L［J］. Journal of Crops,2012, 38(5): 914-920)
［16］文浩,艾辛,蒋建雄,等采用SSR分子标记鉴定荻与南荻F1杂交种的真实性［J］.草原与草坪,2013,33(4):7-11(Wen H, Ai X, Jiang J Q, et al The species distribution of scarab beetle of Gansu province［J］. Grassland and Turf,2013,33(4):7-11)
［17］薛丹丹,郭海林,郑轶琦,等结缕草属植物杂交后代杂种真实性鉴定—SRAP分子标记［J］.作物学报, 2009, 18(2):72-79(Xue D D, Guo H L, Zheng Y Q, et al Hybrid identification of progenies of Zoysia crosses by SRAP marker［J］. Acta Agronomica Sinica, 2009, 18(2):72-79)
［18］陈健文, A J Phillip,劳方业,等应用ISSR标记鉴定斑茅BC2杂种真实性［J］.中国糖料, 2008(1): 1-3(Chen J W, A J Phillip, Lao F Y, et al Identification of BC2 progeny from Saccharum officinarum ×Erianthus arundinaceus by ISSR markers［J］. Sugar Crops of China, 2008(1): 1-3)
［19］李爱贤,刘庆昌,王庆美,等利用 SRAP 标记构建甘薯分子连锁图谱［J］.作物学报, 2010, 36(8): 1286-1295(Li A X, Liu Q C, Wang Q M, et al Establishment of molecular linkage maps using SRAP markers in sweet potato［J］. Acta Agronomica Sinica, 2010, 36(8): 1286-1295)
［20］胡凤荣,何国仁,王斐,等利用 ISSR 分子标记方法鉴定风信子杂种后代［J］.分子植物育种,2015, 13(6):1336-1342(Hu F R, He G R, Wang F, et al The identification of the hyacinth hybrid progeny by the method of ISSR molecular marker［J］. Molecular Plant Breeding,2015, 13(6):1336-1342 )
［21］李巧燕,林瑞庆,朱兴全SRAP分子标记及其应用概述［J］.热带医学杂志,2006,6(4):467-469(Li Q Y, Lin R Q, Zhu X Q Summary of SRAP molecular markers and its application［J］. Journal of Tropical Medicine,2006,6 (4):467-469)
［22］盖树鹏,盖伟玲,黄进勇SSR 与 SRAP 标记在玉米品种鉴定中的比较研究［J］.植物遗传资源学报,2011,12(3):468-472(Gai S B, Gai W L, Huang J Y Comparison of SSR and SRAP marker for varieties identification in maize (Zea maysL．) ［J］. Journal of Plant Genetic Resources,2011,12(3):468-472)
［23］樊洪泓,李廷春,邱婧,等药用石斛遗传多样性的SRAP标记研究［J］. 中国中药杂志,2008,33(1):6-10(Fan H H, Li T C, Qiu J, et al Studies on genetic diversity of medicinal Dendrobium by SRAP［J］. China Journal of Chinese Materia Medica,2008,33(1):6-10)
［24］李仁伟,王晨,戴思兰,等菊花品种表型性状与 SRAP 分子标记的关联分析［J］.中国农业科学,2012,45(7):1355-1364(Li R W, Wang C, Dai S L, et al The association analysis of phenotypic traits with SRAP markers in chrysanthemum［J］. Scientia Agricultura Sinica,2012,45(7):1355-1364)?
［25］王芳,徐翔,李领川,等大豆种质资源SRAP分子标记中的引物筛选［J］. 生物技术通报，2012(7):84-87(Wang F, Xu X, Li L C, et al Primer screening on germplasm resources of Glycine max (L)Merr with SRAP molecular marker［J］. Biotechnology Bulletin，2012(7):84-87)

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Last Update: 2017-05-14