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Functional Prediction and Expression Analysis of GmMP in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2017年01期
Page:
28-32
Research Field:
Publishing date:

Info

Title:
Functional Prediction and Expression Analysis of GmMP in Soybean
Author(s):
LI Dong-mei;  ZHANG Ke-xin; LI Win-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education / Key Laboratory of Northeast Soybean Biology and Genetic Breeding Agriculture Ministry / Northeast Agricultural University, Harbin 150030, China
Keywords:
Soybean;  GmMP gene;  Auxin;  Bioinformatics
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2017.01.0028
Abstract:
Abstract:GmMP gene sequence was obtained by searching the homologous gene of AtMP in the soybean genome database. Bioinformaties analysis of amino acid sequence encoded by GmMP and promoter sequences showed that the full-length CDS sequence was 2 802 bp, encoding 933 amino acids protein. Structure domains analysis indicated that GmMP contained B3 and AUXIN RESPONSE FACTOR domain, belong to B3 and AUXIN RESPONSE superfamily. Cis-elements associated with hormones, stresses, light responses, circadian rhythms and transcription factors binding were existed in the predicted promoter region. Phylogenetic analysis results suggested that MP was conserved in the leguminous plants. Results of tissue specific analysis revealed that expression level of GmMP was the lowest in leaves, the highest in SAM, it was speculated to participate in the auxin metabolic pathways.

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Last Update: 2017-03-14