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Title:: MsDREB1 Overexpression Improves Tolerance to Salt Stress in Transgenic Glycine max L.

Author(s):: LI Da-hong; JIANG Bing-shen; WU Hai-yan; LI Hong-yan; （College of Biology and Food Engineering,Huanghuai University，Zhumadian 463000，China）

Keywords:: Soybean; MsDREB1; rd29A promoter; Salt tolerance

PACS:: -

DOI:: 10.11861/j.issn.1000-9841.2017.01.0017

Abstract:: Transcriptional regulation is one of the important factors in plant adaption to abiotic stress. Transcription factors play a critical role in regulating downstream genes expression in stress signaling pathway and the plant acclimation to adverse environments. Among transcription factors involved in abiotic stress response, DREB represents a large protein family which displays diverse roles in various biological processes. In this study, MsDREB1 was cloned into the 35S promoter and the rd29A promoter. The two vectors were transferred into soybean genome by Agrobacterium mediated. The transgenic plants were detected by Southern blot. Difference in gene expression of 15 day-old seedlings under 200 mmol·L^-1NaCl stress conditions was analyzed by RT-PCR in different time, and the content of chlorophyll, MDA content, SOD, H₂O₂, relative root length and relative length of the above-ground parts of plants were determined. The results showed that there were significant differences in the regulation of MsDREB1 expression between the two promoters. MsDREB1 gene was overexpressed under the regulation of CaMV-35S promoter, but the expression was lower under the regulation of rd29A promoter under non stress. Under salt stress, the expression level of rd29A:MsDREB1 was higher than that of 35S:MsDREB1. Overexpression of MsDREB1inhibited the normal growth of plant. Both of the two transgenic lines had a certain ability to salt tolerance, but there were some differences. The effect of salt resistance of rd29A: MsDREB1 was more obvious. The chlorophyll content, proline content, SOD activity of rd29A:MsDREB1 were significantly higher than those of 35S: MsDREB1, while H2O2 and MDA content were significantly lower than that of 35S:MsDREB1 overexpression. Therefore, MsDREB1 functioned as a transcript regulator contributing to osmotic regulation in soybean plant under NaCl stress.

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MsDREB1 Overexpression Improves Tolerance to Salt Stress in Transgenic Glycine max L.(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

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