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A Method for Rapid Extraction of DNA from Soybean Cyst Nematode(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年06期
Page:
1043-1046
Research Field:
Publishing date:

Info

Title:
A Method for Rapid Extraction of DNA from Soybean Cyst Nematode
Author(s):
LIAN Yun1 FENG Wen-qiang12 LEI Chen-fang1 LI Jin-ying1 WU Yong-kang1 WANG Ting-feng1 LU Li-li1 LU Wei-guo1
(1. Institute of Industrial Crops, Zhengzhou National Subcenter for Soybean Improvement/Key Laboratory of Oil Crops in Huang-huaihai Plains, Ministry of Agriculture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2.State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China)
Keywords:
Soybean Soybean cyst nematode DNA extraction Race
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.06.1043
Abstract:
In this paper, we reported a method for rapid extraction of DNA from soybean cyst nematode(SCN). It is difficult and time consuming to obtain high quality DNA by using bacterial genomic DNA extraction kit for the special growth environment of SCN.Three methods for extraction of DNA were compared in this study, including Bacterial genomic DNA extraction kit, San Prep column plasmid DNA Kit and CTAB method.Results showed that DNA isolated by improved San Prep column plasmid DNA Kit was high quality with time saving. We verified the quality by amplification sequence effectively from rDNA-ITS region. The method could be used for DNA obtaining from cyst and the later molecular manipulations of genes cloning.

References:

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Last Update: 2016-12-09