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Molecular Cloning and Preliminary Functional Prediction of GmAYI in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年06期
Page:
1037-1042
Research Field:
Publishing date:

Info

Title:
Molecular Cloning and Preliminary Functional Prediction of GmAYI in Soybean
Author(s):
ZHOU Shu-li ;  LI Dong-mei ; YANG Yu-ying;  ZHANG Wei-na;  LI Wen-bin;  ZHANG Bin-bin
（Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry，Northeast Agricultural University, Harbin 150030, China）
Keywords:
Soybean;  GmAYI gene;  Correlation;  Quantitative PCR
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.06.1037
Abstract:
The primers were designed according to the conserved sequence of Arabidopsis At1G74730 gene.The GmAYI gene was cloned from soybean by RT-PCR.The GmAYI gene domain and the promoter element were analyzed.The results showed that the GmAYI gene of soybean was located on chromosome 4, which contained transmembrane domain and belonged to membrane protein.The promoter region of the gene contained a hormone-related response element and a cis-acting element necessary for endosperm expression. The results suggested that the gene may be involved in the regulation of soybean growth， development and environmental stress response process. The transcript levels of GmAYI gene in 14 soybean cultivars were quantitatively analyzed.Based on the correlation analysis of GmAYI gene transcripts in 14 soybean cultivars with seeds number per plant, seeds weight per plant, 100-seed weight and plant height, the expression of GmAYI gene was negatively correlated with 100-seed weight, suggested that the GmAYI gene may be involved in the regulation of soybean yield-related traits.

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Last Update: 2016-12-09