|Table of Contents|

Drug Carrier Study of Soybean Protein Isolation(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年06期
Page:
1008-1012
Research Field:
Publishing date:

Info

Title:
Drug Carrier Study of Soybean Protein Isolation
Author(s):
LI Bing-zhang1;  WANG Han1;  GUAN Lu-yao1;  NIU Bao-long1;  2
（1. College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024,China; 2. Key Laboroatory of Interface Science and Engineering in Advanced Materials, Taiyuan University of Technology, Ministry of Education, Taiyuan 030024, China）
Keywords:
Soy protein isolated;  Curcumin;  Drug delivery;  Vacuum freeze-drying;  Cytotoxicity
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.06.1008
Abstract:
To study the drug carrying ability of soy protein isolated(SPI), novel soy protein isolated (SPI)based sponges containing curcumin were prepared by a freeze drying method. The morphology and particle size of soy protein isolated-curcumin nanocomposite(SPI-Cur) was characterized by transmission electron microscopy(TEM) and Nanotrac wave. Then, the drug loading rate, encapsulation efficiency and release behavior of SPI-Cur nanoparticles were measured by high performance liquid chromatography (HPLC). Finally, in vitro cytotoxicity of the Cur micelles and SPI-Cur micelles were investigated by a standard MTT assay. The results showed that SPI achieved good surface coating out of the Cur, the surface roughness of Cur increased and the surface potential rised after coated with SPI, the Cur of SPI-Cur could release steadily and slowly, the prepared SPI-Cur presented drug loading and entrapment efficiency of 3.08% and 33.90%. By means of the MTT assay, it was found that the TE-1 cell viability was related to the reaction time and drug concentration. When 4.0 μg·mL-1 Cur was incubated with TE-1 cells for 24 h, the cell viability declined significantly. As an edible carrier, the development of this subject has greatly broadened its application on medical areas.

References:

［1］李玉珍,肖怀秋,兰立新.大豆分离蛋白功能特性及其在食品工业中的应用［J］. 中国食品添加剂,2008, 1(21): 121-124. (Li Y Z, Xiao H Q, Lan L X. Functional characterizations of SPI and its applications in food industry［J］. China Food Additives, 2008, 1(21): 121-124.)

［2］顾振宇,江美都,付道才,等.大豆分离蛋白乳化性的研究［J］. 中国粮油学报,2000, 15(3):32-34. (Gu Z Y, Jiang M D, Fu D C, et al. Study on emulsifying properties of soy protein isolate［J］. Journal of the Chinese Cereals and Oils Association, 2000, 15(3): 32-34.)
［3］Li M M, Xiao Y, Chen Y, et al. Soy protein-modified waterborne polyurethane biocomposites with improved functionality［J］. RSC Advances, 2016, 6(16): 12837-12849.
［4］王欣,乔玲.大豆分离蛋白的营养、功能特性及应用研究［J］. 农业科技与装备, 2013(5): 55-56. (Wang X, Qiao L. Study on the nutrition, functional properties and application of soy protein isolate［J］. Agricultural Science & Technology and Equipment, 2013(5): 55-56.)
［5］Mackay J A, Chen M N, Mcdaniel J R, et al. Self-assembling chimeric polypeptide-doxorubicin conjugate nanoparticles that abolish tumours after a single injection ［J］. Nature Materials, 2009, 8(12): 993-999.
［6］Sungwon K, Shi Y Z, Ji Y K, et al. Overcoming the barriers in micellar drug delivery: Loading efficiency, in vivo-stability, and micelle-cell interaction［J］. Expert Opinion on Drug Delivery, 2010, 7(1): 49-62.
［7］刘红艳,王海燕,叶松,等.姜黄素药理作用及其机制研究进展［J］. 中国现代医学杂志, 2012, 22(6):48-51. (Liu H Y, Wang Hai-yan, Ye S,et al. Research progress of curcumin pharmacological action and mechanism［J］. China Journal of Modern Medicine, 2012, 22 (6): 48-51.)
［8］Sunagawa Y, Katanasaka Y, Hasegawa K, et al. Clinical applications of curcumin［J］. Pharmanutrition, 2015, 3(4): 131-135.
［9］Dong-Chan K, Sae -Kwang K, Jong -Sup B. Anticoagulant activities of curcumin and its derivative［J］. BMB Reports, 2012,45(4): 221-226.
［10］郑施施,李星熠,王增寿.PCL-PEG-PCL载姜黄素纳米粒子的制备以及体外药物释放的考察［J］. 中国现代应用药学, 2012, 29(6): 513-516.(Zheng S S,Li X Y, Wang Z S. Curcumin loaded PCL-PEG-PCL nanoparticle:Preparation,characterization and in vitro release study［J］. Chinese Journal of Modern Applied Pharmacy, 2012, 29(6): 513-516.)?
［11］Acartürk F, Takka S. Calcium alginate microparticles for oral administration: II. Effect of Formulation factors on drug release and drug entrapment efficiency［J］. Journal of Microencapsulation, 1999, 16(3): 291-301.
［12］Guan T T, Miao Y Q, Xu L S, et al. Injectable nimodipine-loaded nanoliposomes: Preparation, lyophilization and Characteristics［J］. International Journal of Pharmaceutics, 2011, 410(1): 180-187.
［13］李敏,郭爱洁,张骁,等.姜黄素共聚物胶束的制备与体外细胞毒评价［J］. 现代生物医学进展, 2016, 16(3): 427-430. (Li M, Guo A J, Zhang X, et al. Preparation and in vitro cytotoxicity of curcumin-loaded polymeric［J］. Progress in Modern Biomedicine, 2016, 16(3): 427-430.)
［14］张晓燕,李倩,孙建绪,等.纳米效应对难溶性药物阿瑞匹坦体内外性质的影响［J］. 中国药学杂志, 2014, 49(14): 1226-1232.(Zhang X Y, Li Q, Sun J X, et al. Influences of nanometer effects on the characters of water-insoluble drug aprepitant in vivo and in vitro［J］. China Pharmaceutical Journal, 2014, 49(14): 1226-1232.)
［15］朱佳,轩亚茹,张春春,等.姜黄素复方脂质体的制备及质量评价［J］.中国实验方剂学杂志, 2015, 21(19): 19-23.(Zhu J,Xuan Y R,Zhang C C, et al. Preparation and quality evaluation of Curcumin-quinacrine Liposomes［J］. Chinese Journal of Experimental Traditional Medical Formulae,2015, 21(19): 19-23.)?
［16］Gong C, Deng S, Wu Q, et al. Improving antiangiogenesis and antitumor activity of curcumin by biodegradable polymeric micelles［J］. Biomaterials, 2013, 34(4): 1413-1432.
［17］Gao X, Zheng F, Guo G, et al. Improving the anti-colon cancer activity of curcumin with biodegradable nano-micelles ［J］. Journal of Materials Chemistry B, 2013, 1(42): 5778-5790.

Memo

Memo:
-
Last Update: 2016-12-09