Isolation and Molecular Identification of an Endophytic Bacteria from Soybean(PDF)

¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2016Äê06ÆÚ

Page:

997-1001

Research Field:

Publishing date:

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Title:

Isolation and Molecular Identification of an Endophytic Bacteria from Soybean

Author(s):

WANG Bo; ZHOU Jian-nan;  HUANG Zhong-qin;  WANG Xing;  DING Zhen-qian;  CHANG Yong;  ZHOU Xing-gen

£¨Xuzhou Academy of Agricultural Sciences, Xuzhou 221131, China£©

Keywords:

Paenibacillus polymyxa;  Antibiotic activity;  Soybean

PACS:

-

DOI:

10.11861/j.issn.1000-9841.2016.06.0997

Abstract:

In this experiment, an endophytic bacteria XZ-2 with broad spectrum antimicrobial activity was isolated from soybean plant. Antibiotic activity assay showed that XZ-2 had strong antibiotic ability against 8 phytopathogenic fungi, and the diameters of inhibition zone were larger than 20 mm.The growth curves of XZ-2 were drawn by spectrophotometric method, the result showed shaking XZ-2 in liquid LB medium for 12 h could basically reach the logarithmic growth phase, the growth curve drawing for XZ-2 may be useful for fermentation conditions optimization in further study-16SrDNA fragment was amplified by PCR and the phylogenetic tree was constructed by software MEGA 6.0, and then XZ-2 was identified as Paenibacillus polymyxa. This study showed that XZ-2 had brilliant biological control application prospect

References:

£Û1£ÝChi E H, Suk Y K, Jeong M P. Biocontrol activity of Paenibacillus polymyxa AC-1 against Pseudomonas syringae and its interaction with Arabidopsis thaliana£ÛJ£Ý.Microbiological Research, 2016, 185: 13-21.

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