|Table of Contents|

Identification of Red Crown Rot of Soybean Caused by Calonectria ilicicola in Zhejiang Province, China(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年06期
Page:
986-991
Research Field:
Publishing date:

Info

Title:
Identification of Red Crown Rot of Soybean Caused by Calonectria ilicicola in Zhejiang Province, China
Author(s):
GAI Yun-peng;  HUANG Zi-lin;  CHEN Hao-hao;  ZHOU Qiao-li;  MA Hai-jie;  LI Hong-ye
（Institute of Biotechnology, Gollege of Agriculture of Biotechnology, Zhejiang University, Hangzhou 310058, China）
Keywords:
Soybean;  Red crown rot;  Calonectria ilicicola;  Pathogen identification
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.06.0986
Abstract:
Red crown rot (RCR) is one of worldwide fungal diseases in soybean ［Glycine max(L) Merr］. It causes yield loss and seed quality deficiency seriously. The objective of this study is to identify the pathogen causing stem rot on soybean in Zhejiang province, and to provide an IPM strategy for disease control.Typical symptoms of the disease were red discoloration at the base of the soil line with numerous reddish perithecia on the lesion surface. Diseased samples were collected from Tonglu County, Zhejiang province and ascospore suspensions were placed on potato dextrose agar (PDA) plate. One representative isolate ZJHZ01 was chosen for morphological and molecular study.Pathogenicity was verified by conidial suspension inoculation on the basal stems of soybean cultivars. The colonial morphology was basically white, and the colonies tend to change into brown.Numerous yellowish-brown microsclerotia and chlamydospores were consistently formed on colonies. Orangeto-reddish perithecia had formed in old cultures of PDA, V8 and carnation leaf agar. Based on the morphology and pathogenicity analysis, the strain ZJHZ01 was primarily identified as Calonectria ilicicola. Boedijin & Reitsma. In addition, the molecular identification of representative strain ZJHZ01 was conducted, and the TUB, HIS and EF1α gene were amplified and sequenced and compared with sequences deposited in GenBank. The NJ phylogenetic trees were constructed by comparison with strain ZJHZ01 and reference strains. Comparing with other known .Calonectria sp in GenBank, the three genes of ZJHZ01 shared high sequences homology with GDBL01 at a level of 99% similarity Identification of the pathogen was based on morphology of colony and conidial, pathogenicity and phylogenetic analysis. This is the first report of red crown rot of soybean caused by C-ilicicola in Zhejiang Province, China.

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Last Update: 2016-12-09