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Soybean GmRAV Gene Involved in BR Signaling Pathway and Analysis on Recombinant Protein Solubility(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年06期
Page:
928-931
Research Field:
Publishing date:

Info

Title:
Soybean GmRAV Gene Involved in BR Signaling Pathway and Analysis on Recombinant Protein Solubility
Author(s):
YANG Chun-liang1;  SUN Hao-hang1;  ZHANG Han-shuo1;  ZHAO Lin2
（1.Basic Medical Science School, Harbin Medical University, Harbin 150081, China; 2.Key Laboratory of Soybean Biology in Chinese Ministry of Education Northeast Agricultural University/Key Laboratory of Soybean Biology and Breeding (Genetics) of Chinese Agriculture Ministry，Harbin 150030, China）
Keywords:
Soybean; GmRAV protein;  Prokaryotic expression;  Solubility analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.06.0928
Abstract:
Soybean DN42 was sprayed by epiBL to study the effect of BR on GmRAV expression by real-time quantative PCR Meantime, T5 generation GmRAVox tobaccos were treated with epiBL to study if GmRAV was involved in BR signaling to inhibit the plant growth and development.The results showed that the GmRAV mRNA expression level was inhibited by BR, and the exogenous epiBL addition did not promote GmRAVox tobacco stem elongation，which indicated that GmRAV might be a negative regulator in BR signaling pathway. Furthermore, soybean GmRAV was constructed into prokaryotic expression vector pET28a and the recombinant plasmid pET28a-GmRAV was transformed into E.coli BL21 (DE3) to get the expression protein for further study． The bacterial was induced by 0.5mmol·L-1?IPTG in two different temperature 37℃ and 28℃. The result indicated that an 40 kDa recombinant protein was all expressed with the treatment of different temperature by SDS-PAGE．The lower temperature could increase protein solubility．

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Last Update: 2016-12-09