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Title:: Cloning and Functional Analysis of GsAP1 in Wild Soybean

Author(s):: GUO Wen-ya; CUI Yan-mei; YU De-yue; HUANG Fang; （Soybean Research Institute, Nanjing Agricultural University/State Key Laboratory of Crop Genetics & Germplasm Enhancement/National Center for Soybean Improvement/Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Nanjing 210095, China）

Keywords:: Wild soybean; Floral development; AP-1; Transcription factor; Functional analysis

PACS:: -

DOI:: 10.11861/j.issn.1000-9841.2016.06.0919

Abstract:: AP-1 plays an important role in controlling floral organ formation and development. In this report, we isolated an AP₁like gene, designated GsAP1, from the wild soybean flowers. The fragment was 711 bp and contained an open reading frame coding a polypeptide of 237 amino acids. Expression of GsAP1 was analyzed by the method of real-time PCR. The result showed that GsAP1 only expressed in flowers; in the four whorls of floral organs, expression of GsAP1 was prominent in sepals while very weak in the petals, not in stamens and capels; and increased during the course of seed development. Subcellular localization assay suggested that GsAP1 was localized in nucleus. The transcriptional activation ability of GsAP1 protein was confirmed by the yeast assay system. Further we found that GsAP1 caused early flowering when ectopically expressed in tobacco. The mechanism of GsAP1 in soybean was discussed in this paper, which provided molecular resources and theoretical basis for the breeding of soybean varieties.

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Cloning and Functional Analysis of GsAP1 in Wild Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

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