|Table of Contents|

A Study on Sample Preparations for Proteomics of Soybean Seeds(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年05期
Page:
810-817
Research Field:
Publishing date:

Info

Title:
A Study on Sample Preparations for Proteomics of Soybean Seeds
Author(s):
LIN Yang-jie1; 2; 3 ;  ZHAO Ming1;  YANG Sheng-chao1;  PAN Ying-hong2; 3
（1.Institute of agriculture and biotechnology, Yunnan Agricultural University, Kunming 650201, China; 2.The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3.Crop Science Institute of Chinese Academy of Agricultural Sciences, Beijing 100081, China）
Keywords:
Soybean seed;  Proteomics;  Sample preparation;  Nano LC-MS/MS
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.05.0810
Abstract:
By using pheno meth anolammonium acetate, TCA acetone or urea thiourea methods, seed proteins of soybean Zhonghuang 13 were extracted at first and second step. In order to explore the efficient methods of preparing sample for proteomics of soybean seeds.The protein extraction obtained by urea thiourea method at first step and the protein extraction obtained by pheno meth anolammonium acetate method at second step, and the first step extracted protein, which was separated further by one-dimensional electrophoresis, were analyzed with nano LC-MS/MS respectively.The results showed that the protein yield of first and second extractions obtained by urea thiourea method and following by pheno meth anolammonium acetate method reached 29.18% and 4.08% respectively, and was the highest among extractions obtained by other combined methods.A total of 2 246 protein groups was identified from three replicate samples of first and second extraction, and there were significant differences between them.Average 1 167 protein groups were identified from the sample extracted by urea thiourea, and average 1 868 protein groups from the urea thiourea extracted sample which were separated further by one-dimensional electrophoresis.The results indicated that the effective sample preparation and pre-separation could enhance the yields and identification of the protein from soybean seeds.

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Last Update: 2016-09-25