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The Analysis of Key Gene of Soybean Isoflavone Synthesis Response to Aphid Feeding Defense(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年05期
Page:
800-804
Research Field:
Publishing date:

Info

Title:
The Analysis of Key Gene of Soybean Isoflavone Synthesis Response to Aphid Feeding Defense
Author(s):
LI Na;  YU Xi-sen;  LI Qi;  LI Han-zhe;  LI Yang;  XU Ting-ting;  MENG Fan-li
（1.Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin 150030, China; 2. Key Laboratory of Soybean Biology of Chinese Education Ministry, Northeast Agricultural University, Harbin 150030, China）
Keywords:
Soybean;  Isoflavones;  Key Genes;  Soybean Aphid;  Defense Response
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.05.0800
Abstract:
In order to elucidate isoflavone mechanisms of the response to aphid in the soybean plants, different-resistant soybean varieties PI567598B (high), Suinong 30 (medium), Dongnong 51(low) and Williams 82 (flu), were inoculated by soybean aphid for 0, 7, 14 and 21 days.Using qPCR, the expression patterns of key enzyme genes were analyzed in response to aphid feeding defense, including Phenylalanine ammonia lyase (PAL), Flavanone hydroxylase -3-3 (F3H), Isoflavone synthase gene 1 (ISF1) and Isoflavone synthase gene 2 (ISF2).The results showed leaves from PI567598B had higher -PAL, F3H, ISF1 and ISF2 expression than Williams 82 without aphid attack. The PAL gene in PI567598B and Suinong 30 was increased significantly after inoculated 14 d and reached highest level at 21 d. The F3H gene was increased rapidly at 7 d and then showed a decreasing trend; the IFS2 gene was increased rapidly and the highest in 24 h.The PAL, IFS2 and F3H genes in Dongnong 51 were not induced significantly after inoculated 7 d.The PAL and IFS2 genes in Williams 82 were not induced significantly and lower than other three lines even after being damaged by aphid, while gene expression of F3H decreased after inoculation.The expression of IFS1 gene was not induced in four lines after inoculation. These result showed that the expression level of isoflavone key enzyme gene were higher and longer in resistant varieties than that in susceptible soybean varieties, which was consistent with its resistance differences.

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Last Update: 2016-09-25