|Table of Contents|

Lignin Content and Expression of Key Biosynthetic Genes in Soybean upon Infection by P sojae(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年05期
Page:
789-794
Research Field:
Publishing date:

Info

Title:
Lignin Content and Expression of Key Biosynthetic Genes in Soybean upon Infection by P sojae
Author(s):
GAN Shu-ping;  YAN Qiang;  CUI Xiao-xia;  XUE Dong;  ZHAO Jin-ming;  GUO Na;  WANG Hai-tang;  XING Han
（Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvement/Key Laboratory for Biology and Genetic Improvement of Soybean(General)，Ministry of Agriculture/National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, China）
Keywords:
Soybean(Glycine max);  Lignin;  PRR resistance;  Gene expression;  Correlation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.05.0789
Abstract:
To explore the variation tendency of soybean lignin content and key biosynthetic genes expression in the progress of soybean and Phythophthora sojae-interaction and the relationship between them.The lignin contents and key biosynthetic genes expression of Williams roots were analyzed at 6, 8, 10 and 15 days post inoculation(dpi) with P sojae P6497 isolate. The results showed that the lignin content in infected tissues showed a higher accumulations rate when compared with uninfected control. The expression analysis of the 9 key biosynthenic genes exhibit that PAL, C4H and F5H were significantly up-regulated in infected samples than that in control at all selected time points, CCoAOMT was significantly higher at 8, 10, 15 dpi, 4CL showed more than 100 folds higher than control at 10 and 15 dpi, CAD and CCR were significantly higher at 8 and 10 dpi respectively, the remain two genes(C3H and COMT) showed no significantly difference between inoculated and control samples.The further analysis found that the expression of C4H, 4CL and CCR variation tendency in inoculated samples were significantly positive correlated with lignin content. The above results demonstrated that the content of soybean lignin was induced by P sojae- infection, implying that lignin may participate in the interaction between soybean and P sojae, and C4H, 4CL and CCR gens plays a crucial role in lignin synthesis induced by P sojae in soybean.

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Last Update: 2016-09-25