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Cloning and Characterization of GmNAC115 Gene in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年05期
Page:
754-759
Research Field:
Publishing date:

Info

Title:
Cloning and Characterization of GmNAC115 Gene in Soybean
Author(s):
NI Zhi-yong;  YU Yue-hua;  CHEN Quan-jia;  QU Yan-ying
（College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China）
Keywords:
Soybean;  GmNAC115;  Cloning;  Transcriptional activation
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.05.0754
Abstract:
NAC(NAM, ATAF, and CUC)domain proteins play important roles in plant development and stress responses.A gene coding for NAC, designated as GmNAC115 was isolated from soybean. The full length GmNAC115 cDNA is 1 383 bp, including an ORF of 912 bp. This cDNA sequence encoded a polypepide of 303 amino acid residues with a predicted molecular mass of 34-278 kD and a basic isoelectric point of 8.37, and a highly conserved NAM domain in the encoded putative protein.The genomic DNA of GmNAC115 contains three exons and two introns. Phylogenetic analysis indicated that GmNAC115 clustered with PvNAC. Transcriptional activation ability assay showed that the GmNAC115 transcription factor had transcriptional activation functions SDS-PAGE indicated that the best expression quantity of the p-ET-28a-GmNAC115 was induced with 1.0 mmol.L-1?IPTG for 2 h at 37℃.Tissue-specific expression profiles showed that GmNAC115 expressed almost in all detected tissues, and its expression level was relatively high at root and low at seed.

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Last Update: 2016-09-25