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Expression Analysis of GmWRKY20 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年05期
Page:
748-753
Research Field:
Publishing date:

Info

Title:
Expression Analysis of GmWRKY20 in Soybean
Author(s):
NING Wen-feng1;  PANG Tian1;  YANG Yan-ling1;  ZHANG Chao1; 2;  BAI Xi1
College of Life Science, Northeast Agricultural University, Harbin 150030, China; 2.Qiqihar Horticultural Research Institute, Qiqihar 161006, China
Keywords:
soybean;  GmWRKY20 gene;  Expression analysis;  Abiotic stress
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.05.0748
Abstract:
GsWRKY20 was identified from Glycine soja as an abiotic stress related gene in our previous studies. Overexpression of GsWRKY20 could enhance the drought tolerance of transgenic Arabidopsis or Alfalfa, and promote the flowering of transgenic Arabidopsis. GsWRKY20 encodes a WRKY.type transcription factor, with 99% identity to GmWRKY20 from Glycine max. In order to fully investigate the role and the possible molecular mechanism of GmWRKY20 in soybean, the expression of GmWRKY20 in cultivated soybean was fully studied by real-time RT-PCR. The results showed that the expression of GmWRKY20 did not show obvious differences among different soybean cultivars, and high expression of GmWRKY20 was observed before flowering, suggesting that the high expression of GmWRKY20 might play an important role in starting flowering. GmWRKY20 did not response to photoperiod but response to multiple abiotic stresses at transcript level.

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Last Update: 2016-09-25