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Cloning and Bioinformatics Analysis of GmNup96 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年05期
Page:
736-741
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatics Analysis of GmNup96 in Soybean
Author(s):
JI Dan-dan1; XIAO Long1; SUN Pei-yuan2; LIU Chun-yan3; FU Yong-fu4; CHEN Qing-shan1
（1.College Agriculture,Northeast Agricultural University,Harbin 150030,China; 2.Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar 161041,China; 3.Land Reclamation Research and Breeding Centre of Heilongjiang,Harbin 150050,China; 4.Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100000,China）
Keywords:
Soybean;  GmNup96;  Gene cloning;  Bioinformatics analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.05.0736
Abstract:
The cDNA of GmNup96 gene in Tianlong 1 was cloned, and its bioinformatics character was analyzed.The results showed that the gene encoded a 116-199 7 kDa protein with 1 022 amino acids ,which was a hydrophilic protein without signal peptide, and the GmNup96 protein contained a conserved autoproteolytic peptidase domain in the N terminus. Through the secondary structure prediction, GmNup96 protein contained alpha-helix(46.87%), beta turn ( 9.78%), extended strand(17.03%) and random coil (26.32%) Phylogenetic analysis of GmNup96 and its homologs with different plant species were conducted by the software MEGA 5-1.The results showed that GmNup96 from Glycine max were near with-Glycine soja, Phaseus vulgaris, Vigna radiata var. radinta and Vigna angularis in genetic relationship

References:

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Last Update: 2016-09-24