|Table of Contents|

Resistance Candidate Genes GmMIOX4 Gene Expression Analysis,Cloning and Over Expression Vector Constructing(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年05期
Page:
730-735
Research Field:
Publishing date:

Info

Title:
Resistance Candidate Genes GmMIOX4 Gene Expression Analysis,Cloning and Over Expression Vector Constructing
Author(s):
LIU Ting1; YANG Ruo-wei1; WANG Xue-min1; YU Bai-shuang2; WANG Hui1; DUAN Yu-xi3
（1.Shenyang Agricultural University, College of Bioscience and Biotechnology, Shenyang 110866, China; 2.Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; 3.Shenyang Agricultural University, College of Plant Protection, Shenyang 110866, China）
Keywords:
:GmMIOX4 gene;  Soybean cyst nematode;  Real-time quantitative PCR;  Clone;  Over expression vector
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.05.0730
Abstract:
Two kinds of different resistance soybean(Huipizhiheidou and Liaodou 15) were inoculated with SCN3 in the research.By real-time quantitative PCR method, GmMIOX4 gene expression changes were identified in early period and complete life cycle of SCN3.Real-time quantitative PCR results showed that: Under SCN3 infection, the relative expression of GmMIOX4 gene in roots of Liaodou 15 increased until 25 dpi after inoculation, the relative expression of GmMIOX4 gene in roots of Huipizhiheidou reached the highest level in 10 dpi, GmMIOX4 gene was induced and played an important role.Host’s nutrients was absorbed by SCN through syncytial to maintain normal growth and development period at 10 dpi, and this gene expression is induced, the gene can changes syncytial structure, inhibit nematode development, and thus played a disease-resistant effect. Total RNA of Huipizhi Heidou was used as template, 939 bp CDS of GmMIOX4 genezone was obtained by RT-PCR According to sequencing result, pGEM-MIOX4 over expression vector was cloned, it laid the foundation for further study of GmMIOX4 gene function.

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Last Update: 2016-09-24