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New Method for the Selection of Explants in the Agrobacterium Mediated Cotyledon Nodes Transformation in Soybean (Glycine max L)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年05期
Page:
723-729
Research Field:
Publishing date:

Info

Title:
New Method for the Selection of Explants in the Agrobacterium Mediated Cotyledon Nodes Transformation in Soybean (Glycine max L)
Author(s):
WANG Shuang1; 2;  GUO Bing-fu2;  ZHANG Li-juan2;  GUO Yong2;  JIN Long-guo2;  YANG Hui2;  QIU Li-juan2
（1.College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 2.The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/MOA Key Lab of Soybean Biology(Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China）
Keywords:
Explant status;  Transient expression;  Germination time
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2016.05.0723
Abstract:
The genetic transformation of soybean is one of the difficulties in plant transgenic and Agrobacterium-mediated cotyledonary node transformation method is the most commonly used in soybean genetic transformation. Interaction between target cells and Agrobacterium is the key of this method and explant status is an important factor that influence sensitivity and regeneration ability.In this study, the explant was divided into 6 types according to the color and status of cotyledon and hypocotyl. Transient transformation was carried out with zhonghuang 10, Jack and Shuilizhan and the index of transient expression efficiency of GUS gene was used to explore the best time to prepare explants. The results showed that the explants status were positively correlated with germination time and leaf length and had effect on transient expression efficiency. The best status of different receptors was different. The best periods of Jack and Zhonghuang 10 were HG, HG+CG and CG, the best periods of Shuilizhan was YL. Those cnclusions could provide a reference for the standardization of soybean genetic transformation system.

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Last Update: 2016-09-24