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Protective Effect of Soy Isoflavones on Oxidative Damage of Liver Cells Inducedby Hydrogen Peroxide(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年04期
Page:
687-690
Research Field:
Publishing date:

Info

Title:
Protective Effect of Soy Isoflavones on Oxidative Damage of Liver Cells Inducedby Hydrogen Peroxide
Author(s):
YIN Xue-zhe1; JIN Yan-hua1; HE Xin2; JIN Hai-nan2; QUAN Ji-shu2
1. Yanbian University Hospital，Yanji 133002，China; 2. Medical College of Yanbian University，Yanji 133000，China
Keywords:
Soy isoflavones;  H2O2;  Oxidative damage;  Liver cells
PACS:
Ｒ285. 5
DOI:
10.11861/j.issn.1000-9841.2016.04.0687
Abstract:
The protective effect of soy isoflavones on oxidative damage induced by hydrogen peroxide( H2O2) in liver cells wasinvestigated. Chang Liver cells were exposed to H2O2 and used as the model of oxidative damage. Then the antagonist effect ofsoy isoflavones was detected by pretreatment with 10，20 and 40 mg·L － 1 of soy isoflavones prior to H2O2 challenge. Cell viabilitywas evaluated with MTT assay. The activities of LDH，ALT，AST in culture fluid，and levels of superoxide dismutase( SOD) ，reduced glutathione ( GSH) ，and malondialdelyde( MDA) of liver cells were measured by the microplate tecnique.The protein expression of nuclear factor erythroid2-related factor 2( Nrf2) was determined with the immunoblotting technique.The results showed that 300 μmol·L － 1 H2O2 inhibited cell viabilities，elevated the leakage of LDH，ALT and AST to culturefluid，reduced the levels of SOD and GSH，and increased MDA content in Chang Liver cells. Soy isoflavones did not exert atoxic effect on Chang Liver cells at the concentrations of 10 － 40 mg·L － 1 ，while H2O2 increased oxidative stress and decreasedthe cell viability. Compared with the model group，cell viabilities of soy isoflavone group increased in a dose-dependent manner.Soy isoflavones also reduced the leakage of LDH，ALT and AST to culture fluid，reduced MDA content，and increasedthe levels of SOD and GSH. The administration with soy isoflavones up-regulated the protein expression of Nrf2. Taken together，soy isoflavones have the protective effect on cellular oxidative damage induced by H2O2 in liver cells.

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Last Update: 2016-08-23