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Ｒesearch of the Promoter of Soybean GmGBP1 Induced by Hormone and ExogenousEnvironment(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年04期
Page:
683-686
Research Field:
Publishing date:

Info

Title:
Ｒesearch of the Promoter of Soybean GmGBP1 Induced by Hormone and ExogenousEnvironment
Author(s):
DING Fu-quan; DU Hai-long; SUO Yi-ning; YANG Xue; LI Min-min; ZHANG Ke-xin; ZHAO Lin; LI Wen-bin
Agricultural College of Northeast Agricultural University /Key Laboratory of Soybean Biology in Chinese Ministry of Education /Key Laboratory of SoybeanBiology and Breeding ＆ Genetics of Chinese Agriculture Ministry，Harbin 150030，China
Keywords:
Arabidopsis;  GmGBP1 promoter;  GUS staining
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0683
Abstract:
The expression pattern of the GmGBP1 promoter under hormone and environment were obtained． The GmGBP1 promoterwas cloned by PCＲ and the fusion expression vector was constructed，including the GmGBP1 promoter and the reportergene GUS． The fusion expression vector was used to infect Arabidopsis thaliana，and T3 generation of transgenic Arabidopsiswas obtained，then the GUS staining was used to research the expression pattern of the GmGBP1 promoter in Arabidopsis thaliana．The results showed that the expression of the GmGBP1 promoter was regulated by GA，SA，drought and high temperature，consistent with the predictions of GmGBP1 promoter element by bioinformatics analysis． Our experiment provide theoreticalbasis for the cultivation of resistant crops in the future．

References:

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Last Update: 2016-08-23