|Table of Contents|

Multiplex PCR for Detection of Roundup-Ready Soybean GTS40-3-2(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年04期
Page:
666-671
Research Field:
Publishing date:

Info

Title:
Multiplex PCR for Detection of Roundup-Ready Soybean GTS40-3-2
Author(s):
YIN Quan1LI Yi2SONG Jun1WANG Dong1ZHANG Fu-li1LIU Wen-juan1CHANG Li-juan1LIU Yong3
?1. Center of Analysis and Testing,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China; 2. Sichuan Minzu College,Kangding 626001,China; 3. Institute of Plant Protection,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China
Keywords:
Multiplex PCR Soybean GTS40-3-2 Detection
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0666
Abstract:
In order to establish a multiplex PCR ( polymerase chain reaction) method detection for Roundup-Ready soybeanGTS40-3-2,the soybean endogenous reference gene ( Lectin) ,exogenous gene CaMV 35S promoter,CP4-EPSPS and NOSterminator were used as detection parameters. The optimization results showed that the optimum annealing temperature of multiplePCR was 58℃,and the optimum final concentration of primers( μmol·L - 1 ) ratio is 0. 1∶ 0. 2∶ 0. 2∶ 0. 2. The multiplexPCR method is confirmed to be reliable through known samples. And it is proved that the multiplex PCR system is an effectivemethod for detection Roundup-Ready soybean GTS40-3-2.

References:

1] 张海波. 适合转基因大豆GTS40-3-2 复合PCR 检测标准分子研制及番茄内标准基因国际协同验证[D]. 上海: 上海交通大学,2008: 25. ( Zhang H B. A novel plasmid molecule for duplexquantitative PCR analysis of roundup ready soybean and internationalvalidation of tomato endogenous reference gene [D].Shanghai: Shanghai Jiaotong University,2008: 25. )

[2] 李信申,何红,胡汉桥,等. 抗草甘磷转基因大豆多重PCR检测及假阳性问题探讨[J]. 广东海洋大学学报,2008,28( 6) : 66-71. ( Li X S,He H,Hu H Q. Multiplex PCR detectionof transgenetic soybean resistant to glyphosate and discussion offalse positive results[J]. Journal of Guangdong Ocean University,2008,28( 6) : 66-71. )
[3] 中华人民共和国农业部农业转基因生物安全管理办公室.2004-2011 年进口用作加工原料的农业转基因生物审批情况[EB/OL]. ( 2012-02-03) . [2015-06-18]. http: / /www. moa.gov. cn /ztzl /zjyqwgz /spxx /201202 /P020130618408715248212.pdf. ( The agricultural genetically modified organisms safety managementoffice of MOA. The approval of imported agriculture GMOused as processing raw materials from 2004 to 2012 in china [EB/OL]. ( 2012-02-03) . [2015-06-18]. http: / /www. moa. gov. cn /ztzl /zjyqwgz /spxx /201202 /P020130618408715248212. pdf. )
[4] 芦春斌,周文,刘标. 喂食转基因大豆对子代雄鼠生殖系统的影响[J]. 大豆科学,2013,32 ( 1 ) : 119-123. ( Lu C B,Zhang W,Liu B,et al. Effects of transgenic soybean on reproductivesystem in male mice [J]. Soybean Science,2012,31( 2) :292-294. )
[5] Domingo J L. Health risks of GM foods: Many opinions but fewdata [J]. Science,2000,288( 5472) : 1748-1749.
[6] 金芜军,贾士荣,彭于发. 不同国家和地区转基因产品标识管理政策的比较[J]. 农业生物技术学报,2004,2( 1) : 1-7.( Jin W J,Jia S R,Peng Y F,et al. Comparison of labeling policyof genetically modified products in different countries and territories[J]. Journal of Agricultural Biotechnology,2004,12( 1) : 1-7. )
[7] 中华人民共和国农业部. 农业转基因生物标识管理办法[EB/OL]. ( 2001-12-19) . [2015-06-18]. http: / /www. gov. cn /gongbao/content /2002 /content_61835. htm. ( Ministry of Agricultureof the People’s Republic China. Identification measures for theadministration of agricultural genetically modified organisms [EB/OL]. ( 2001-12-19 ) . [2015-06-18]. http: / /www. gov. cn /gongbao /content /2002 /content_61835. htm. )
[8] 黄昆仑,罗云波. 用巢式和半巢式PCR 检测转基因大豆Roundup Ready 及其深加工食品[J]. 农业生物技术学报,2003,11( 5) : 461-466. ( Huang K L,Luo Y B. Detecting genet4期尹全等: 多重PCR 检测抗草甘膦大豆GTS40-3-2 671ically modified soybean roundup ready ingredient in foodstuffs bynested PCR and semi-nested PCR[J]. Journal of Agricultural Biotechnology,2003,11( 5) : 461-466. )
[9] 尹全,宋君,刘勇. 生物技术作物食品检测技术研究进展[J]. 江西农业学报,2010,22( 5) : 135-137. ( Yin Q,Song J,Liu Y. Research progress in detection techniques for food from biotechcrops [J]. Acta Agriculturae Jiangxi,2010,22( 5) : 135-137. )
[10] 张富丽,雷绍荣,刘勇. 转基因作物及加工品检测技术概述[J]. 生物技术通讯,2009,20( 5) : 733-737. ( Zhang F L,LeiS R,Liu Y. Detection methods for transgenic trops and its processedgoods [J]. Letters in Biotechnology,2009,20( 5) : 733-737. )
[11] 徐景升,余爱丽,姚伟,等. 利用MPCR 技术快速检测进口大豆转基因背景[J]. 江西农业大学学报,2005,27 ( 2) : 262-264,269. ( Xu J S,Yu A L,Yao W,et al. Rapid Detection ofimported genetically modified soybean ( Glycine max) with MPCR[J]. Acta Agriculturae Universitatis Jiangxiensis,2005,27( 2) :262-264,269. )
[12] 何玮玲,张驰,杨静,等. 食品中4 种肉类成分多重PCR 的快速鉴别方法[J]. 中国农业科学,2012,45( 9) : 1873-1880.( He W L,Zhang C,Yang J,et al. A quick multiplex PCR methodfor the identification of four meat ingredients in food products[J]. Scientia Agricultura Sinica,2012,45( 9) : 1873-1880. )
[13] 范宏英,吴清平,吴若菁,等. 饮用水中5 种致病菌多重PCR技术检测研究[J]. 微生物学通报,2005,32 ( 3) : 102-107.( Fan H Y,Wu Q P,Wu R J,et al. Study on detection of fivekindsof pathogenic bacteria in drinking water by multiplex PCR[J]. Microbiology,2005,32( 3) : 102-107. )
[14] Chamberlain J S,Gibbs R A,Rainer J L,et al. Detection screeningof the duchenne muscular dystrophy locus via multiplex DNAimplication [J]. Nucleic Acids Research,1988,16: 1141-1156.
[15] Fore V T,Pinto A D,Martino C,et al. A general multiplex PCRassay for the general detection of genetically modified soya andmaize [J]. Food Control,2005,l6( 6) : 535-539.
[16] Germini A,Zanetti A,Salati C,et al. Development of a seventarget multiplex PCR for the simultaneous detection of transgenicsoybean and in feeds and foods [J]. Journal of Agricultural andFood Chemistry,2004,52 ( 11) : 3275-3280.
[17] GMO detection method database ( GMDD) [EB/OL]. [2015-06-18]. http: / /gmdd. shgmo. org /event /view/88.
[18] 中华人民共和国农业部. NY/T 675-2003: 转基因植物及其产品检测大豆定性PCP 方法[S]. 北京: 中国标准出版社,2003.( Ministry of Agriculture of the People’s Republic China. NY/T675-2003: Detection of genetically modified plant organisms andderived products-Qualitative PCR methods for soybeans [S]. Bei-Jing: China standard publishing house,2003. )[19] 中华人民共和国农业部. 农业部1782 号公告-3-2012: 转基因植物及其产品成分检测调控元件CaMV 35S 启动子、FMV 35S启动子、NOS 启动子、NOS 终止子和CaMV 35S 终止子定性PCR 方法[S]. 北京: 中国标准出版社,2012. ( The agriculturedepartment announcement no. 1782-3-2012. Detection of geneticallymodified plants and derived products-Qualitative PCR methodsfor the regulatory elements CaMV 35S promoter,FMV 35Spromoter,NOS promoter,NOS terminator and CaMV 35S terminator[S]. BeiJing: China standard publishing house,2012. )
[20] 中国国家标准化管理委员会. GB/T 19495. 6-2004: 转基因产品检测基因芯片检测方法[S]. 北京: 中国标准出版社,2004. ( Standardization Administration of the People’s Republic ofChina. GB/T 19495. 6-2004: Detection of genetically modified organismsand derived products gene chip detection [S]. Beijing:China Standard Publishing House,2004. )
[21] 赵红庆,苑锡铜,黄留玉. 多重PCR 技术在病原检测中的应用[J]. 生物技术通讯,2007,8 ( 5) : 863-866. ( Zhao H Q,Yuan X T,Huang L Y. Application of multiplex PCR in detectionof pathogens [J]. Letters in Biotechnology,2007,8 ( 5 ) : 863-866. )
[22] Henegariu O,Heerema N A,Dlouhy S R,et al. Multiplex PCR:Critical parameters and step-by-step protocol[J]. Biotechniques,1997,23 ( 3) : 504-511.

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Last Update: 2016-08-23