|Table of Contents|

Development of dcELISA Method for Ｒapid Detection Glycinin of Soybean

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年04期
Page:
660-665
Research Field:
Publishing date:

Info

Title:
Development of dcELISA Method for Ｒapid Detection Glycinin of Soybean
Author(s):
ZHANG Shi-yao; ZHAO Yuan; BAO Nan; NA-Ｒen-Gao-Wa; LIU Dan-dan
College of Animal Science and Technology，Jilin Agricultural University /Key Laboratory of Animal Production，Product Quality and Security，Ministry ofEducation /Jilin Key Laboratory of Animal Nutrition and Feed Science，Changchun 130118，China
Keywords:
Glycinin;  ELISA;  Soybean antigen;  Allergy
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0660
Abstract:
The present study presents a direct enzyme-linked immunosorbent assay( ELISA) that allows for the detection oftrace amount of glycinin in soybean. In this assay，rabbit anti-glycinin polyclonal antibody( Pab) was used as primary antibodyand goat anti-mouse igG-horseradish peroxidae( HＲP) was used as secondary antibody. Twenty-five samples collected in partsof Jilin province were preliminary tested by this detection method and compared with competitive ELISA method. Ｒesultsshowed amounts of glycinin in about 64% samples detected by the dcELISA were significantly higher than that detected bycompetitive ELISA and the coincidence rate of the standard deviation( SD) was more than 72%. The method showed a goodsensitivity and specificity and was suitable for quick and large quantitative detection of glycinin in food and feed industry.

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Last Update: 2016-08-23