|Table of Contents|

Characterization of Ammonia Oxidizing Archaea Community and Its DrivingFactors in the Ｒhizosphere of Continuous Cropping Soybean in Black Soil(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年04期
Page:
620-625
Research Field:
Publishing date:

Info

Title:
Characterization of Ammonia Oxidizing Archaea Community and Its DrivingFactors in the Ｒhizosphere of Continuous Cropping Soybean in Black Soil
Author(s):
CHEN Xue-li; LI Wei-qun; WANG Ying; WANG Yu-feng; WANG Xiao-jun
( Institute of Soil Fertilizer and Environment Ｒesources，Heilongjiang Academy of Agricultural Sciences /Key Laboratory of Soil Environment and Plant Nutritionof Heilongjiang Province /Fertilizer Engineering Technology Ｒesearch Center of Heilongjiang Province，Harbin 150086，China)
Keywords:
Black soil;  Soybean continuous cropping;  Ｒizosphere;  Diversity of soil microorganism community;  Ammonia oxidizingarchaea
PACS:
S154. 3
DOI:
10.11861/j.issn.1000-9841.2016.04.0620
Abstract:
Continuous cropping resulted in the changing of metabolism process in the rhizosphere of soybean，while the communitycomposition of nitrogen related functional genes were always used to evaluate the response of the nitrogen conversion capabilityof soil to continuous cropping system. Ammonia oxidizing was the first step in the course of nitrogen transformation andalso the limited speed step，so that ammonia oxidizing bacteria( AOB) and ammonia oxidizing archaea were always consideredthe driver of the ni trogen transformation process. In this study，the methods of qPCＲ and Illumina Miseq sequencing were usedto analyze the ammonia oxidizing archaea( AOA) community composition and quantities in rhizosphere of soybean based on thesoybean continuous cropping located observation in black soil. The results showed that the abundance of Archaea amoA gene inSC2 and SC17 were lower than that in other treatments. AOA in the rhizosphere of soybean belongs to two phylums，Crenarchaeotaand Thaumarchaeota，and still have unclassified AOA accounted for 42%. The correlated analysis between AOA compositionand environment factors indicated that the AOA compositions of MS ( maize-soybean rotation) and SC2 were positiverelated to the contents of total nitrogen and total carbon，then that of treatments with more than continuous cropping years of 4showed positive related to pH and C/N ratio.

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Last Update: 2016-08-23