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Effects of Genistein Combined Polyunsaturated Fatty Acids on Lipid MetabolicGene Expression of MCF-7 Cells(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年04期
Page:
587-592
Research Field:
Publishing date:

Info

Title:
Effects of Genistein Combined Polyunsaturated Fatty Acids on Lipid MetabolicGene Expression of MCF-7 Cells
Author(s):
DU Pei; SHEN Hong-yi; LI Zhong-ping; LIU Xia; CHEN Gao-min; XIE Yan
( College of Public Health，Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China)
Keywords:
MCF-7 cells;  Gentistein;  Polyunsaturated fatty acids
PACS:
Ｒ737. 9
DOI:
10.11861/j.issn.1000-9841.2016.04.0587
Abstract:
The effect of gentistein which is the anticancer component in soy，combined different ratios polyunsaturated fattyacids( PUFAs) on proliferation and lipid metabolic gene expression of MCF-7 cells was studied. MCF-7 was cultured with differentconcentration gentistein( GEN) and docosahexaenoic acid( DHA) . According to the effect concentration of DHA，we setlinoleic acid( LA) and mixed PUFAs’ concentration，which was 10∶ 1 LA to DHA. Then we combined GEN with DHA，LAand LA∶ DHA ( 10∶1) respectively，texted the survival rate and detect mＲNA expression of peroxisome proliferators-activatedreceptor γ( PPAＲγ) ，5-lipoxygenase ( 5-LOX) and cyclooxygenase-2 ( COX-2) with qPCＲ. The results showed that highconcentration of GEN and DHA could inhibit proliferation of MCF-7 cells and the effect enhanced with time. However，lowconcentration of GEN could promote the cells proliferation mildly. LA and 10∶ 1 PUFAs promote the cells proliferation，butcell’s proliferation was inhibited when we added GEN. GEN could reduce 5-LOX mＲNA level of MCF-7 cell which was underthe effect of LA. Meanwhile it inhibits proliferation of MCF-7 cell through promoting PPAＲγ mＲNA level and inhibiting COX-2 mＲNA level when the cell is cultured with GEN combined 10∶1 LA∶ DHA. GEN can inhibit the promoting effect of high ratioω-6 PUFAs’ proliferation of breast cancer cells. The mechanism is mainly through improving the expression of PPAＲγ mＲNAand inhibiting COX-2 mＲNA. The synergistic effect of GEN and PUFAs on inhibiting the proliferation of breast cancer cells，may be the reason for the anti breast cancer of soy.

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Last Update: 2016-08-22