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Molecular Cloning and Gene Diversity Analysis of Gene AK in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2016年04期
Page:
574-580
Research Field:
Publishing date:

Info

Title:
Molecular Cloning and Gene Diversity Analysis of Gene AK in Soybean
Author(s):
YU Yan1; TANG Jing-xian1; JIANG Wei2; JIANG Hong-wei3; QIU Hong-mei4; LIU Chun-yan3; CHEN Qingshan2; HU Guo-hua3
( 1. College of Biology and Food，Changchun University of Science and Technology，Changchun 130600，China; 2. College of Agriculture，Northeast AgriculturalUniversity，Harbin 150030，China; 3. The Crop Ｒesearch and Breeding Center of Heilongjiang Land Ｒealamation，Harbin 150090，China;4. Soybean Ｒesearch Institute，Jilin Academy of Agricultural Sciences，Changchun 130033，China)
Keywords:
Soybean;  Aspartic acid metabolism;  Aspartate kinase( AK) ;  Gene diversity
PACS:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.04.0574
Abstract:
AK gene was cloned from soybean，a cDNA product about 1 690 bp was amplified from the total ＲNA of soybeanleaves by reverse transcription PCＲ( ＲT-PCＲ) with specific primers designed with the full length sequence of AK gene fromNCBI． The cDNA of 19 varieties were amplified with the same way. The PCＲ products were cloned，sequenced，and analyzedwith bioinformatics software. The results showed that there were two mutant alleles of nucleotide sequences in different soybeanvarieties，the first was the sequence which had a nucleotide substitution，the second was the sequence which had an insertionof 6 nucleotides between 1 369 and 1 374 bp. There were only one mutant allele of amino acid sequences in protein level，which was an insertion of 2 amino acids at 456 － 457 amino acid residue. The structure of AK was completed，the conserveddomains of AA_kinase，Carbamate kinase-like，Aspartate kinase，and ACT were found in protein of AK. The gene diversity analysisof gene AK in soybean was studied for the first time in this research. The results provided a theoretical basis for thestudy of other related enzymes in the aspartate pathway of soybean，and also laid a good foundation for the research and applicationof AK gene in plant genetic engineering and other fields.

References:

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Last Update: 2016-08-22